| 来源 |
用途 |
交叉反应性 |
理论分子量 |
实际分子量 |
| Rabbit |
WB, IHC |
H, M, R |
46kDa |
47kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB |
IP |
IF |
IHC |
ICC |
FC |
ChIP |
ChIP-seq |
| 1:500-1:2000 |
- |
- |
1:100-1:200 |
1:100-1:200 |
- |
- |
- |
抗体详细信息如下::
| About this Antibody |
| Name |
VEGFC Rabbit Polyclonal Antibody |
| Category |
Rabbit Polyclonal Antibody (pAb); Primary antibody |
| Isotype |
IgG |
| Purification method |
Affinity purification |
| Positive samples |
BT-474, MCF7, Mouse spleen, Rat liver |
| Cellular location |
Secreted |
| Customer validation |
- |
| About the Immunogen |
| Immunogen |
A synthetic peptide of human VEGFC |
| Sequence |
- |
| Gene ID |
7424 |
| Swiss Prot |
P49767 |
| Synonyms |
VEGFC; Flt4-L; LMPH1D; VRP; vascular endothelial growth factor C |
| Category |
Angiogenesis; Growth Factors |
| Background |
Vascular endothelial growth factor (VEGF) is a highly specific mitogen for vascular endothelial cells. VEGF and its close relatives VEGF-B, -C and -D form a subfamily within PDGF family of growth factors, which belongs to the cysteine knot class of cytokines. Five VEGF isoforms of 121, 145, 165, 189 and 206 amino acids (VEGF121–206) are generated as a result of alternative splicing from a single VEGF gene. The various VEGF forms bind to three tyrosine-kinase receptors, VEGFR-1, VEGFR-2 and VEGFR-3 which are expressed almost exclusively in endothelial cells. VEGFR-2 is the main angiogenic signal transducer for VEGF, while VEGFR-3 is specific for VEGF-C and -D and is necessary and sufficient for lymphangiogenic signaling. However, upon proteolytic processing VEGF-C and -D gain the ability to also bind and activate VEGFR-2. Guided by the binding properties of the ligands, the VEGFRs are able to form both homodimers and heterodimers. Receptor dimerization is accompanied by activation of receptor kinase activity leading to receptor autophosphorylation. Phosphorylated receptors recruit interacting proteins and induce downstream signaling. Recently, tumor therapies based on neutralizing anti-VEGF antibodies and small molecule tyrosine kinase inhibitors targeting VEGFRs have been developed. These new strategies for tumor treatment show the clinical relevance of inhibiting VEGF signal transduction pathways that are exaggerated in pathological angiogenesis. |
关键字： ANTI-VEGF-C ;VEGF-C ;抗体;
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