ANTI-AKT1

靶点:详见说明
浓度:1mg/ml
应用范围:WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test1
CC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
宿主:Rabbit
适应物种:Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit Guinea Pig
标记物:可现标
克隆性:单克隆
保存条件:Store at -20%C for one year.
形态:冻干粉
亚型:IgG
免疫原:KLH conjugated synthetic peptide derived from human
规格:50ul-100ul
Anti-AKT1抗体Myc-Tag单克隆抗体
size
200ug/100ug/50ug
Source
Mouse
Immunogen
Synthetic Peptide
Purification
The antibody was afinity-purified from mouse ascites by afinity-chromatography using specific immunogen.
Specificity
The antibody can detect Myc fusion proteins.
Formulation
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Concentration
1 mg/ml
Storage 1 Stability
-20*C/1 year
Reactivity
N/A
Applications
WB, IP, IF
Dilution
Anti-AKT1抗体WB: 1:5000 IP: 1:200 IF: 1:1000
Myc-Tag Monoclonal Antibody Images
IP antibody use: 3ug Flag Mouse lgG1 per ml Lysate, WB 1: 5000 1) untransfected 293 cell lysate 2) transfected 293 cell
lysate with Myc-Tag fusion protein 3) IP (transfected 293+ normal Mouse lgG+Protein G agarose) 4) IP (transfected
293+anti- C-Myc m
IF analysis of 293 cells transfected with a Myc-tag protein,1:2000 dilution (blue DAPI, red anti-MyC)
1ug Myc fusion protein+ Primary antibody dilution at 1) 1:5000 2) 1:10000
Anti-AKT1抗体注意事项:
1抗体保存原则:避免反复冻融
2:短期保存的抗体:如抗体几周内就会使用，收到后推荐在2-8°C保存，2-8*C短暂保存数周对抗体活性没有影响。
3.长期保存的抗体:如抗体需超过1个月后才会使用，请酌情分装后于-20°C保存。
4建议:将抗体保存在冰箱里层，避免温度变化造成反复冻融。
绝对避免将抗体保存在自动除霜冰箱中。
反复冻融过程中，冰晶会对抗体的空间结构造成破坏，导致蛋白变性形成多聚体，从而降低抗体的结合能力，加快抗体降解速度。