ANTI-HISTONE H3 (DI METHYL K4)抗体

Anti-Methyl-Histone H3 (di K4) Rabbit Monoclonal AntibodyRabbitMouse, Rat, HumanSynthetic peptide within Human Histone H3 aa 1-100 (di methyl K4).PepArr, ICC/IF, ChIP, IHC-P, WB

ChIP - Anti-Histone H3 (di methyl K4) antibody [EPR17707] - ChIP Grade (ab176878)Chromatin was prepared from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab176878 (blue), and 20µl of Anti Rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach). Primers and probes are located in the first kb of the transcribed region.

Histone H3 is a histone protein. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. They play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Histone H3 is characterized by a main globular domain and a long N-terminal tail. It is involved with the structure of the nucleosomes of the 'beads on a string' structure. There are five main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone H3 is the most extensively modified of the five histones. In the emerging field of epigenetics, it is thought that Histone H3 plays a key role, cause its sequence variants and variable modification states function in the dynamic and long term regulation of genes.ANTI-HISTONE H3(DI METHYL K4)抗体是一类可以特异性结合Anti-HISTONE H3(DI METHYL K4)的多克隆抗体，主要用于检测Anti-HISTONE H3(DI METHYL K4)的Western Blot、IHC-P、IF、ELISA、Co-IP等多种免疫学实验。双抗体夹心法测定标本中Anti-HISTONE H3(DI METHYL K4)水平。用纯化的Anti-HISTONE H3(DI METHYL K4)抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入Anti-HISTONE H3(DI METHYL K4)，再与HRP标记的Anti-HISTONE H3(DI METHYL K4)抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的Anti-HISTONE H3(DI METHYL K4)呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中Anti-HISTONE H3(DI METHYL K4)浓度。