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Anti-pan-AKT抗体

Anti-pan-AKT抗体 更多供应商
公司名称: 北京冬歌博业生物科技有限公司  
联系电话: 4008558271
产品介绍: 英文名称:Anti-pan-AKT antibody
公司名称: 江西江蓝纯生物试剂有限公司  
联系电话: 021-54720761
产品介绍: 英文名称:Anti-pan-AKT antibody
公司名称: 上海机纯实业有限公司  
联系电话: 13524933993 021-54720761
产品介绍: 英文名称:Anti-pan-AKT antibody
公司名称: 北京百奥创新科技有限公司  
联系电话: 15710099392 010-57187533
产品介绍: 英文名称:Anti-pan-AKT antibody
Anti-pan-AKT抗体专题
Anti-pan-AKT抗体的应用
Anti-pan-AKT抗体是一类可以特异性结合AKT的单克隆抗体,主要用于检测AKT的Western Blot、IHC-P、IF、ELISA、Co-IP等多种免疫学实验。
2020/8/12 8:39:49
Anti-pan-AKT抗体生产厂家及价格列表
Anti-pan-AKT抗体_江西江蓝纯生物试剂有限公司
价格:询价
2020/10/29 07:31:50
Anti-pan-AKT抗体_上海机纯实业有限公司
价格:询价
2020/10/29 07:31:50
Anti-pan-AKT抗体
描述宿主种属反应性验证的应用品牌示例背景下游应用简介检测原理 专题
中文名称:Anti-pan-AKT抗体
中文同义词:ANTI-PAN-AKT抗体
英文名称:Anti-pan-AKT antibody
英文同义词:Anti-pan-AKT antibody
CAS号:
分子式:
分子量:0
EINECS号:
相关类别:
Mol文件:Mol File
Anti-pan-AKT抗体
Anti-pan-AKT抗体 性质
Anti-pan-AKT抗体 用途与合成方法
描述兔多克隆抗体to pan-AKT
宿主Rabbit
种属反应性Mouse, Rat, Human
验证的应用IHC-P, WB, ICC/IF
品牌示例

Immunocytochemistry/ Immunofluorescence - Anti-pan-AKT antibody (ab8805)

The Akt antibody (ab8805) is used at 1/80 on cultured neonatal rat cardiomyocytes that express a nuclear-targeted Akt construct. The green is Akt antibody, the red is Texas-red™ phalloidin that labels actin filaments.

背景v-akt murine thymoma viral oncogene homolog 1 (AKT1), or protein kinase B-alpha (PKB-ALPHA) is a serine-threonine protein kinase, belonging to the Protein Kinase Superfamily. AKT1 is a major mediator of the responses to insulin, insulin-like growth factor 1 (IGF1), and glucose. AKT1 also plays a key role in the regulation of both muscle cell hypertrophy and atrophy. AKT1 activity is required for physiologic cardiac growth in response to IGF1 stimulation or exercise training. In contrast, AKT1 activity was found to antagonize pathologic cardiac growth that occurs in response to endothelin 1 stimulation or pressure overload. AKT1 selectively promotes physiological cardiac growth while AKT2 selectively promotes insulin-stimulated cardiac glucose metabolism. AKT1 deletion prevented tumor initiation as well as tumor progression, coincident with decreased Akt signaling in tumor tissues. AKT1 is the primary Akt isoform activated by mutant K-ras in lung tumors, and that AKT3 may oppose AKT1 in lung tumorigenesis and lung tumor progression. A number of separate studies have implicated AKT1 as an inhibitor of breast epithelial cell motility and invasion. AKT1 may have a dual role in tumorigenesis, acting not only pro-oncogenically by suppressing apoptosis but also anti-oncogenically by suppressing invasion and metastasis.

下游应用Anti-pan-AKT抗体是一类可以特异性结合AKT的单克隆抗体,主要用于检测AKT的Western Blot、IHC-P、IF、ELISA、Co-IP等多种免疫学实验。
简介蛋白激酶B,即Akt,又称PKB或Rac,在细胞存活和凋亡中起重要作用。胰岛素等生长和存活因子都可以激活Akt信号途径。Akt的Thr308可以被PDK1磷酸化。 PI3 Kinase-Akt信号途径是一条经典的信号途径,LY294002等PI3 kinase的抑制剂抑制PI3 kinase时,通常就会抑制Akt激活。 AKT1基因编码的是丝氨酸/苏氨酸蛋白激酶,可通过磷脂酰肌醇3激酶(PI3K)依赖的机制被胞外信号激活,目前发现AKT家族有三个成员,分别为AKT1/PKBα、AKT2/PKBβ和AKT3/PKBγ。
检测原理双抗体夹心法测定标本中AKT水平。用纯化的AKT抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入AKT,再与HRP标记的AKT抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的AKT呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),通过标准曲线计算样品中AKT浓度。
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