(1S,2R)-FMOC-2-AMINOCYCLOHEXANE CARBOXYLIC ACID synthesis

Yield:-

Steps:

Multi-step reaction with 6 steps
1.1: dichloromethane / 0 - 20 °C / Inert atmosphere
1.2: 36 h
2.1: potassium carbonate / water / Sonication
3.1: Amano Lipase PS from Burkholderia cepacia immobilized on celite / acetone / Inert atmosphere
4.1: ammonium hydroxide / methanol; water / 22 h / Inert atmosphere
5.1: hydrogenchloride; water
6.1: potassium hydrogencarbonate / water; acetone / 48 h

References:

Lengyel, George A.;Frank, Rebecca C.;Horne, W. Seth [Journal of the American Chemical Society,2011,vol. 133,# 12,p. 4246 - 4249]