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ChemicalBook CAS DataBase List germacreneD,1-methyl-5-methylene-8-(1-methylethyl)-1,6-cyclodecadiene

germacreneD,1-methyl-5-methylene-8-(1-methylethyl)-1,6-cyclodecadiene synthesis

6synthesis methods
-

Yield:-

Steps:

Multi-step reaction with 3 steps
1: Escherichia coli hydroxyethylthiazole kinase; ATP; pyruvate kinase / aq. buffer / 16 h / 20 °C / pH 8 / Enzymatic reaction
2: isopentenyl phosphate kinase from Methanocaldococcus jannaschii; ATP; pyruvate kinase / aq. buffer / 16 h / 20 °C / pH 8 / Enzymatic reaction
3: (2E,6E)-FDP synthase from Geobacillus stearothermophilus; (-)-germacrene D synthase from Solidago canadensis; 1,4-dithio-L-threitol; magnesium chloride / aq. buffer / 16 h / 20 °C / pH 8 / Enzymatic reaction

References:

Allemann, Rudolf K.;Benton, Jennifer C. R.;Dunbabin, Alice;Johnson, Luke A.;Mart, Robert J. [Angewandte Chemie - International Edition,2020,vol. 59,# 22,p. 8486 - 8490][Angew. Chem.,2020,vol. 132,# 22,p. 8564 - 8568,5]