SAGITTATOSIDE A synthesis

Yield:-

Reaction Conditions:

with β-dextranase;water in aq. acetate buffer at 60; pH=4.5; for 1 h;Catalytic behavior;Enzymatic reaction;Reagent/catalyst;Temperature;pH-value;Solvent;

Steps:

Screening of enzymes

In the first stage, five commercial enzymes including β-glucosidase, β-dextranase, cellulase, naringinase and glucoamylase were investigated for efficient hydrolysis of epimedin A to sagittatoside A (Figure S1). of Epimedin A and each of these enzymes (12.5~800 μg) was mixed and dissolved in 2 mL of 0.20 M HAc-NaAc buffer (pH 4.5), prior to incubation at 60°C for 1 hr. Then, another 2 mL of MeOH was added into the hydrolysate to denature the enzymes after hydrolysis. The resulting suspension was filtered through a 0.20 μm polytetrafluoroethylene (PTFE) membrane syringe, and subsequent 1 mL of filtrate was collected into clear vial for HPLC-UV analysis after the initial 1 mL was discarded. Then, the enzyme was selected from these five enzymes according to the conversion rate of Epimedin A.

References:

Shen, Yuping;Lu, Yi;Gao, Jing;Zhu, Yeting;Wang, Man;Jing, Shunli;Xu, Lili;Yang, Huan;Jia, Xiaobin [Natural Product Research,2019,vol. 33,# 21,p. 3095 - 3102] Location in patent:supporting information