| Identification | More | [Name]
Xylene Cyanole FF | [CAS]
2650-17-1 | [Synonyms]
ACID BLUE 147
CI 42135
CI 43535
CI NO 42135
CI NO 43535
CYANOL
CYANOL BLUE
CYANOL FF
XC
XYLENE CYANOL
XYLENE CYANOLE FF
XYLENE CYANOL FF
1,3-benzenedisulfonicacid,4-[[4-(ethylamino)-3-methylphenyl][4-(ethylimino)-3
1,3-Benzenedisulfonicacid,4-[[4-(ethylamino)-3-methylphenyl][4-(ethylimino)-3-methyl-2,5-cyclohexadien-1-ylidene]methyl]-,monosodiumsalt
5-cyclohexadien-1-ylidene]methyl]--methyl-monosodiumsalt
Xylene Cyanol FF（Acid blue 147）
Acid Blue 147(42135)
xylene cyanole ff (dye content 75%)
XYLENECYANOL FF, FOR MICROSCOPY, REDOX I NDICATOR
XYLENE CYANOLE FF, DYE CONTENT:*APPROX 7 5% | [EINECS(EC#)]
224-728-5 | [Molecular Formula]
C25H27N2NaO6S2 | [MDL Number]
MFCD00040657 | [Molecular Weight]
538.61 | [MOL File]
2650-17-1.mol |
| Chemical Properties | Back Directory | [Appearance]
dark green or blue-grey crystals | [Melting point ]
295°C | [storage temp. ]
Store at RT. | [solubility ]
H2O: complete1mg/mL, clear, blue | [Colour Index ]
42135 | [form ]
Solid | [color ]
Green | [PH Range]
2.9 (purple) - 4.6 (green)(Methyl Orange - Xylene Cyanol) | [Stability:]
Stable. Moisture-sensitive. Incompatible with strong oxidizing agents. | [Water Solubility ]
Soluble in water. | [CAS DataBase Reference]
2650-17-1(CAS DataBase Reference) | [EPA Substance Registry System]
2650-17-1(EPA Substance) |
| Safety Data | Back Directory | [Hazard Codes ]
Xi | [Risk Statements ]
R36/37/38:Irritating to eyes, respiratory system and skin . | [Safety Statements ]
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice .
S36:Wear suitable protective clothing .
S24/25:Avoid contact with skin and eyes . | [WGK Germany ]
3
| [TSCA ]
Yes | [HS Code ]
32041900 |
| Hazard Information | Back Directory | [Chemical Properties]
dark green or blue-grey crystals | [Uses]
Suitable as a tracking dye for DNA sequencing. | [Uses]
Xylene Cyanol FF is a tracking dye commonly used for nucleic acids. The compound has been used in both electrophoresis polyacrylamide and agarose gels for various evaluations including DNA sequencing. The compound has also been used in a mobility shift analysis of ssDNA. In addition, two highly sensitive spectrophotometric assays have been developed for the detection of platinum (IV) (sc-250738, Platinum(IV) chloride) and Chromium (sc-214714) with the use of Xylene Cyanol FF. As an organic dye-inactivated Sendai virus vaccine, Xylene Cyanol FF displayed strong protection against the virus. | [Preparation]
4-Formylbenzene-1,3-disulfonic acid (1 Moore) and N-ethyl-2-methylbenzenamine (2 Moore) condensation, garnish with heavy chromate salt-oxalic acid oxidation its product. | [General Description]
Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The tracking dye typically migrates with the DNA molecules around 5kb. Xylene cyanol may also be used in loading buffer that facilitates the observation of the sample during gel loading and electrophoresis. | [Biochem/physiol Actions]
Xylene Cyanol FF acts as nucleic acid stain. | [Properties and Applications]
bright blue. Soluble in water to blue.
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Standard
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Light Fastness
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Soaping
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Persperation Fastness
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Oxygen bleaching
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Fastness to seawater
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Fading
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Stain
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Fading
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Stain
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Fading
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Stain
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|
ISO
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2
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2
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3-4
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2-3
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1
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|
2-3
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1
|
|
AATCC
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2
|
4-5
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4-5
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2-3
|
1
|
|
2
|
2
|
|
|
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