| Identification | Back Directory | [Name]
PNGASE F | [CAS]
83534-39-8 | [Synonyms]
PNGASE A
PNGASE F
EC 3.5.1.52
N-GLYCANASE
N-GLYCANASE(R)
GLYCOPEPTIDASE
N-GLYCOSIDASE A
N-GLYCOSIDASE F
GLYCOPEPTIDASE A
GLYCOPEPTIDASE F
PEPTIDE N-GLYCOSIDASE
PEPTIDE-N-GLYCOSIDASE F
N-GLYCOSIDASE A, ALMOND
GLYCOPEPTIDASE FROM ALMONDS
glycopeptidase a from almonds
Native Almonds Glycopeptidase A
PNGase F from Elizabethkingia miricola
PNGASE F RECOMBINANT CHRYSEOBACTERIUM
PNGASE F HIGH CONCENTRATION FROM CHRYSEO
Native Elizabethkingia miricola PNGase F
(PEPTIDE-N4-[N-ACETYL-BETA-GLUCOSAMINYL)]
PNGaseF from Elizabethkingia meningoseptica
Native Flavobacterium meningosepticum PNGase F
Native Elizabethkingia meningoseptica PNGase F
N-Glycosidase F, Elizabethkingia meningosepticum
N-GLYCOSIDASE F, CHRYSEOBACTERIUM MENINGOSEPTICUM
PNGase F from Elizabethkingia miricola, Recombinant
Glycopeptidase F from Elizabethkingia meningoseptica
peptide-N4-(N-acetyl--glucosaMinyl) asparagineaMidase
peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase
PEPTIDE-N4-(ACETYL-BETA-GLUCOSAMINYL)-ASPARAGINE AMIDASE
PNGase F from Elizabethkingia meningoseptica, Recombinant
PNGase F from Flavobacterium meningosepticum, Recombinant
PNGASE (F CHRYSEOBACTERIUM (FLAVOBACTERIUM) MENINGOSEPTICUM)
N-Glycosidase F, Chryseobacterium meningosepticum, Recombinant, E. coli
GLYCOPEPTIDASE F, RECOMBINANT, SOLUTION, FROM FLAVOBACTERIUM MENINGOSEPTICUM
N-linked-glycopeptide-(N-acetyl-β-D-glucosaminyl)-L-asparagine amidohydrolase
pngase f from elizabethkingia (chryseobacterium/flavobacterium) meningsepticum
N-LINKED-GLYCOPEPTIDE-[N-ACETYL-BETA-D-GLUCOSAMINYL]-L-ASPARAGINE AMIDOHYDROLASE
PNGASE FROM ELIZABETHKINGIA (CHRYSEOBACTERIUM/FLAVOBACTERIUM) MENINGSEPTICUM, PROTEOMICS GRADE
N-Glycosidase A, PNGase A, N-linked-glycopeptide-(N-acetyl-β-D-glucosaminyl)-L-asparagine amidohydrolase
PNGASE FROM ELIZABETHKINGIA (CHRYSEOBACTERIUM/FLAVOBACTERIUM) MENINGSEPTICUM, RECOMBINANT EXPRESSED IN ESCHERICHIA CO
N-GlycosidaseF, Peptide N-glycosidase, PNGase F from Chryseobacterium meningosepticum, PNGase F from Flavobacterium meningosepticum
N-Glycanase, N-GlycosidaseF, Peptide N-glycosidase, PNGase F from Chryseobacterium meningosepticum, PNGase F from Flavobacterium meningosepticum | [MDL Number]
MFCD00131222 |
| Hazard Information | Back Directory | [Uses]
Glycopeptidase A from almonds is used for deglycosylation. It catalyzes the removal of N-linked oligosaccharide chains and converts Asn residue to Asp. | [Uses]
PNGase F from Elizabethkingia meningoseptica has been used:
- for de-N-glycosylation of Zika20virus E protein
- to evaluate coxsackievirus and adenovirus receptor glycosylation using CAR-expressing COS cells
- to verify the N-linked glycosylation of MHC class 1 polypeptide-related sequence A (MICA)
| [Uses]
Recombinant PNGase F has been purified by affinity chromatography and dialyzed into a 50% glycerol solution with 10 mM potassium phoosphate pH 7.5 to produce a stable product. The product contains low levels of buffer salts. This highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-terminal 6x histidine fusion tag. PNGase F from Elizabethkingia meningoseptica has been used in deglycosylation assay in human plasma samples and in deglycosylation of chondroitin sulfate proteoglycan. | [General Description]
PNGase F (Peptide N-glycosidase F) cleaves asparagine-linked glycoproteins, which produces carbohydrate-free peptides and detached full-length oligosaccharides. | [Biochem/physiol Actions]
Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain. |
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