| Identification | Back Directory | [Name]
GLYCEROKINASE | [CAS]
9030-66-4 | [Synonyms]
GK
GLK
HK4
HKIV
HHF3
EC 2.7.1.30
GLYCEROKINASE
GLYCEROL KINASE
Glyceric kinase
Glycerin kinase
GK/Glycerokinase
testis specific 2
MEK kinase kinase 3
Glycerol Kinase (GK)
Glycerolkinase,E.coli
GLYCEROL, PCR REAGENT
GLYCEROKINASE USP/EP/BP
glycerokinase from E coli
Glycerokinase, 150 units/Mg
Glycerol phosphotransferase
glycerol kinase from E. coli
Glycerokinase [ >=250 units/mg]
MAPK/ERK kinase kinase kinase 3
Kinase(phosphorylating),glycerol
Glycerokinase from microorganisms
GLYCEROKINASE, FROM MICROORGANISM
glycerolkinasefromcandidamycoderma
ATP: GLYCEROL 3-PHOSPHOTRANSFERASE
Glycerokinase, 150 units/mg protein
glycerokinase from escherichia coli
glycerokinase from cellulomonas sp.
Native Microorganism Glycerol Kinase
Native Cellulomonas sp. Glycerokinase
Native Escherichia coli Glycerokinase
glycerokinase from streptomyces canus
Glycerol Kinase from Escherichia coli
glycerokinase from cellulomonas species
Native Streptomyces canus Glycerokinase
GLYCEROL KINASE FROM E. COLI LYOPH. &
Monoclonal Anti-GCK antibody produced in mouse
Anti-GK, C-Terminal antibody produced in rabbit
GLYCEROKINASE FROM BACILLUS*STEAROTHERMO PHILUS
Native Bacillus stearothermophilus Glycerokinase
Anti-GK3, N-Terminal antibody produced in rabbit
glycerokinase from bacillus*stearothermophilus so
GLYCEROL KINASE FROM CANDIDA MYCODERMA, ~85 U/MG*
Anti-MAP4K3, N-Terminal antibody produced in rabbit
GLYCEROKINASE FROM BACILLUS*STEAROTHERMO PHILUS SOLU
ATP:glycerol 3-phosphotransferase, Glycerol Kinase
ANTI-GLYCEROL KINASE (GPK2)(C-TERMINAL) antibody produced in rabbit
ATP: glycerol 3-phosphotransferase, GK, Glycerokinase from Escherichia coli
Glycerokinase from Escherichia coli,ATP:glycerol 3-phosphotransferase, Glycerol Kinase
Glycerokinase from Cellulomonas sp.,ATP:glycerol 3-phosphotransferase, Glycerol Kinase
Glycerokinase from Streptomyces canus,ATP:glycerol 3-phosphotransferase, Glycerol Kinase
Glycerokinase from Bacillus stearothermophilus,ATP:glycerol 3-phosphotransferase, Glycerol Kinase | [EINECS(EC#)]
232-862-0 | [Molecular Formula]
NULL | [MDL Number]
MFCD00131205 |
| Hazard Information | Back Directory | [Chemical Properties]
Beige powder | [Uses]
Glycerol kinase (glpK) was used to study the effects of pain controlling neuropeptides on human fat cell lipolysis. | [Uses]
Glycerol kinase has been used along with adenosine triphosphate (ATP) for the conversion of glycerol to glycerol-3 phosphate (glycerol-3P) in white adipose tissue (WAT) adipocyte samples by label distribution method. It has also been used along with adenosine triphosphate (ATP) in the derivatization of glycerol to sn-glycerol-3 phosphate (glycerol-3P). | [Uses]
This enzyme is useful for enzymatic determination of glycerol and triglyceride when coupled with glycerol-3-phosphate dehydrogenase (=G-3-P DH, G3D-301), glycerol-3-phosphate oxidase (=G-3-P oxidase, G3O-301, G3O-311, G3O-321) or pyruvate kinase (PYK-301) and lactate dehydrogenase (LCD-209, LCD-211), lipoprotein lipase (LPL-311, LPL-314) in clinical analysis. | [Biochem/physiol Actions]
Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate. | [Purification Methods]
Commercial enzyme has been dialysed against 2mM Hepes, 5mM dithiothreitol and 0.3mM EDTA, followed by several changes of 20mM Hepes and 5mM dithiothreitol prior to storage under N2 at -20o. [Knight & Cleland Biochemistry 28 5728 1989.] The enzyme from pigeon liver is purified by acid-precipitation (acetate buffer at pH 5.1), (NH4)2SO4 fractionation, heat treatment (60o/ 1hour), calcium phosphate gel filtration, a second (NH4)2SO4 fractionation, dialysis, elution of inert proteins and crystallisation. This is done by repeatedly extracting the precipitate from the last step with 0.05M sodium pyrophosphate (pH 7.5) containing 1mM EDTA and 0.2M (NH4)2SO4 is added. Careful addition of solid (NH4)2SO4 to this solution leads to crystallisation of the enzyme. Recrystallisation is repeated. The enzyme is activated by Mg2+ and Mn2+ ions and is most stable in solutions in the pH 4.5-5.5 range. The stability is greatly increased in the presence of glycerol. It has Km for glycerol of 60WM and for ATP 9WM in glycine buffer pH 9.8 and 25o. [Kennedy Methods Enzymol 5 476 1962.] |
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