2-Nitrophenyl-beta-D-galactopyranoside
- CAS No.
- 369-07-3
- Chemical Name:
- 2-Nitrophenyl-beta-D-galactopyranoside
- Synonyms
- ONPG;o-NPG=ONPG;2-Nitrophenyl;ONPG Solution;ONP-BETA-D-GAL;2-Nitrophenyl-beta-D;2-Nitrophenyl-b-D-galactopy;2-Nitrophenyl hexopyranoside;ONP BETA-D-GALACTOPYRANOSIDE;2-Nitrophenyl-β-D-galactoside
- CBNumber:
- CB1366392
- Molecular Formula:
- C12H15NO8
- Molecular Weight:
- 301.25
- MDL Number:
- MFCD00063255
- MOL File:
- 369-07-3.mol
- MSDS File:
- SDS
| Melting point | 186-193 °C (dec.) |
|---|---|
| alpha | -70 º (c=1, H2O) |
| Boiling point | 442.39°C (rough estimate) |
| Density | 1.3709 (rough estimate) |
| refractive index | -68.8 ° (C=1, H2O) |
| storage temp. | 2-8°C |
| solubility | DMSO (Slightly), Water (Slightly, Heated, Sonicated) |
| pka | 12.69±0.70(Predicted) |
| form | Powder |
| color | White to very slightly yellow |
| optical activity | [α]20/D 70±1°, c = 1% in H2O |
| Water Solubility | Soluble in water. |
| BRN | 92207 |
| InChIKey | KUWPCJHYPSUOFW-RMPHRYRLSA-N |
| CAS DataBase Reference | 369-07-3(CAS DataBase Reference) |
| FDA UNII | 8RFX6AT9WP |
| NIST Chemistry Reference | Galactopyranoside, o-nitrophenyl, beta-d-(369-07-3) |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS07 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Signal word | Warning | |||||||||
| Hazard statements | H315-H319-H335 | |||||||||
| Precautionary statements | P261-P264b-P271-P280-P302+P352-P304+P340-P305+P351+P338-P312-P362+P364-P403+P233-P501c | |||||||||
| Hazard Codes | Xi | |||||||||
| Risk Statements | 36/37/38 | |||||||||
| Safety Statements | 22-24/25-36-26 | |||||||||
| WGK Germany | 3 | |||||||||
| F | 3-10-21 | |||||||||
| HS Code | 29400090 | |||||||||
| NFPA 704 |
|
2-Nitrophenyl-beta-D-galactopyranoside price More Price(49)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | 48712-M | o-Nitrophenyl-β-D-galactopyranoside - CAS 369-07-3 - Calbiochem A β-galactosidase substrate for colorimetric and EIA applications; counterpart of widely used pNPP/alkaline phosphatase substrate. | 369-07-3 | 1g | $54.7 | 2024-03-01 | Buy |
| Sigma-Aldrich | 48712-M | o-Nitrophenyl-β-D-galactopyranoside - CAS 369-07-3 - Calbiochem A β-galactosidase substrate for colorimetric and EIA applications; counterpart of widely used pNPP/alkaline phosphatase substrate. | 369-07-3 | 5G | $208 | 2024-03-01 | Buy |
| Sigma-Aldrich | 73660 | 2-Nitrophenyl β-D-galactopyranoside ≥99.0% (HPLC) | 369-07-3 | 1G | $40.3 | 2022-05-15 | Buy |
| Sigma-Aldrich | 73660 | 2-Nitrophenyl β-D-galactopyranoside ≥99.0% (HPLC) | 369-07-3 | 5G | $142 | 2022-05-15 | Buy |
| TCI Chemical | N0418 | 2-Nitrophenyl beta-D-Galactopyranoside [Substrate for beta-D-Galactosidase] >98.0%(HPLC) | 369-07-3 | 1g | $30 | 2021-12-16 | Buy |
2-Nitrophenyl-beta-D-galactopyranoside Chemical Properties,Uses,Production
Description
2-Nitrophenyl-β-D-galactopyranoside (ONPG) is a colorimetric and spectrophotometric substrate for detecting β-galactosidase activity. This compound is usually colorless. However, if β-galactosidase is present, it hydrolyzes the ONPG molecule into galactose and ortho-nitrophenol. The latter compound has a yellow color that can be used to check for enzyme activity utilizing a colorimetric assay (at 420 nm wavelength). β-Galactosidase is required for lactose utilization, so the intensity of the color produced can be used to measure the enzymatic rate. Though ONPG mimics lactose and is hydrolyzed by β-galactosidase, it cannot act as an inducer for the lac operon. Without another lactose analog that can act as an inducer, such as isopropyl β-D-1-thiogalactopyranoside (IPTG), β-galactosidase will not be transcribed and ONPG will not be hydrolyzed.
Chemical Properties
White Crystalline Solid
Uses
2-Nitrophenyl-beta-D-galactopyranoside is a β-Galactosidase substrate for colorimetric and EIA applications; counterpart of widely employed pNPP/alkaline phosphatase substrate.
Definition
ChEBI: A beta-D-galactoside having a 2-nitrophenyl substituent at the anomeric position.
General Description
ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric substrate for β-galactosidase.
Biochem/physiol Actions
β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.
Purification Methods
Purify 2-nitrophenyl--D-galactopyranoside by recrystallisation from EtOH. [Seidman & Link J Am Chem Soc 72 4324 1950, Snyder & Link J Am Chem Soc 75 1758 1953]. It is a chromogenic substrate for -galactosidases [Jagota et al. J Food Sci 46 161 1981]. [Beilstein 17/7 V 52.]
2-Nitrophenyl-beta-D-galactopyranoside Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| CD Chemical Group Limited | +8615986615575 | info@codchem.com | China | 20356 | 58 |
| Yunbio Tech Co.,Ltd. | +86-010-60605551 +86-18046518538 | yunbiochem@126.com | China | 321 | 58 |
| Hebei Mojin Biotechnology Co., Ltd | +8613288715578 | sales@hbmojin.com | China | 12456 | 58 |
| Hebei Yanxi Chemical Co., Ltd. | +8617531190177 | peter@yan-xi.com | China | 5993 | 58 |
| Anhui Ruihan Technology Co., Ltd | +8617756083858 | daisy@anhuiruihan.com | China | 994 | 58 |
| Capot Chemical Co.,Ltd. | 571-85586718 +8613336195806 | sales@capotchem.com | China | 29797 | 60 |
| Henan Tianfu Chemical Co.,Ltd. | +86-0371-55170693 +86-19937530512 | info@tianfuchem.com | China | 21691 | 55 |
| Hangzhou FandaChem Co.,Ltd. | 008657128800458; +8615858145714 | fandachem@gmail.com | China | 9348 | 55 |
| Shanghai Zheyan Biotech Co., Ltd. | 18017610038 | zheyansh@163.com | CHINA | 3620 | 58 |
| career henan chemical co | +86-0371-86658258 | sales@coreychem.com | China | 29914 | 58 |
View Lastest Price from 2-Nitrophenyl-beta-D-galactopyranoside manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2023-10-11 | 2-Nitrophenyl-beta-D-galactopyranoside
369-07-3
|
US $0.00 / KG | 1KG | 99% | 50000KG/month | Hebei Mojin Biotechnology Co., Ltd | |
 |
2023-09-26 | 2-Nitrophenyl-beta-D-galactopyranoside
369-07-3
|
US $40.00-40.00 / kg | 1kg | 0.99 | 10 tons | Hebei Yanxi Chemical Co., Ltd. | |
 |
2023-09-06 | 2-Nitrophenyl-beta-D-galactopyranoside
369-07-3
|
US $0.00-0.00 / KG | 1KG | 99% | 500000kg | Hebei Guanlang Biotechnology Co., Ltd. |
-
- 2-Nitrophenyl-beta-D-galactopyranoside
369-07-3
- US $0.00 / KG
- 99%
- Hebei Mojin Biotechnology Co., Ltd
-
- 2-Nitrophenyl-beta-D-galactopyranoside
369-07-3
- US $40.00-40.00 / kg
- 0.99
- Hebei Yanxi Chemical Co., Ltd.
-
- 2-Nitrophenyl-beta-D-galactopyranoside
369-07-3
- US $0.00-0.00 / KG
- 99%
- Hebei Guanlang Biotechnology Co., Ltd.
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