| Company Name: |
Shenzhen Regent Biochemical Technology Co., Ltd.
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| Tel: |
0755-0755-85201366 18938635012 |
| Email: |
sales@regentsciences.com |
| Products Intro: |
Product Name:phosphoramide mustard
CAS:10159-53-2
Purity:93% Package:50mg;100mg Remarks:wihite solid, for research
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| | phosphoramide mustard Basic information |
| | phosphoramide mustard Chemical Properties |
| Melting point | 115 °C | | Boiling point | 363.5±52.0 °C(Predicted) | | density | 1.474±0.06 g/cm3(Predicted) | | pka | 0.41±0.50(Predicted) |
| Toxicity | LD50 intraperitoneal in mouse: 184mg/kg |
| | phosphoramide mustard Usage And Synthesis |
| Definition | ChEBI: Phosphoramide mustard is a nitrogen mustard and a phosphorodiamide. | | Biological Activity | Phosphoramide mustard is the active metabolite of cyclophosphamide (HY-17420) with antitumor activity. It induces DNA damage. | | in vitro | Phosphoramide mustard causes cytotoxicity through forming cross-linked DNA adducts which inhibit DNA strand separation during replication.
It (3-6 μM; 48 hours) reduces cell viability in rat spontaneously immortalized granulosa cells ( SIGCs).
Phosphoramide mustard (3-6 μM; 24-48 hours) induces DNA adduct formation and ovarian DNA damage.
It (3-6 μM; 24-48 hours) increases DNA damage responses (DDR) gene mRNA expression levels and DDR proteins.
Cell Viability Assay | Cell Line: | SIGCs | | Concentration: | 0.5 μM, 1 μM, 3 μM, 6 μM | | Incubation Time: | 48 hours | | Result: | Reduced ce ll viability at concentrations of 3 μM and higher. | RT-PCR | Cell Line: | SIGCs | | Concentration: | 3 μM, 6 μM | | Incubation Time: | 24 hours, 48 hours | | Result: | Increased DDR gene mRNA expression levels. | Western Blot Analysis | Cell Line: | SIGCs | < /tr> | Concentration: | 3 μM, 6 μM | | Incubation Time: | 24 hours, 48 hours | | Result: | Generally increased DDR pro teins. | | | target | |
| | phosphoramide mustard Preparation Products And Raw materials |
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