延命草素
| 中文名称 | 延命草素 |
|---|---|
| 中文同义词 | 延命草素;延命素;延命素,延命草素 |
| 英文名称 | enmein |
| 英文同义词 | enmein;Isodonin |
| CAS号 | 3776-39-4 |
| 分子式 | C20H26O6 |
| 分子量 | 362.42 |
| EINECS号 | |
| 相关类别 | 中药对照品;对照品;二萜;标准品 -中药标准品 |
| Mol文件 | 3776-39-4.mol |
| 结构式 |  |
延命草素 性质
| 熔点 | 274-276℃ (Decomposition) (ethanol ) |
|---|---|
| 沸点 | 610.3±55.0 °C(Predicted) |
| 密度 | 1.37±0.1 g/cm3(Predicted) |
| 酸度系数(pKa) | 12.66±0.60(Predicted) |
Enmein 从 I. serra 中分离，具有免疫抑制作用。
Enmein shows significant inhibitory effect toward K562 with an IC 50 value ranging from 3.2 μg/ml to 8.2 μg/ml. Enmein (3.13-25 μg/ml; 48 hours) has inhibitory effect on lymphocytes with significant difference within concentration from 6.25 μg/ml to 25 μg/ml. Enmein (5.5-22 μg/ml; 48 hours) causes the percentage of S stage cells decreased from 19% to 6%, causes the percentage of G2/M stage cells to decrease from 2% to 0.4%.
Cell Viability Assay
| Cell Line: | Splenic lymphocyte cells |
| Concentration: | 3.13, 6.25, 12.5, 25 μg/ml |
| Incubation Time: | 48 hours |
| Result: | Exhibited an IC 50 value of 17 μg/ml in splenic lymphocytes. |
Cell Cycle Analysis
| Cell Line: | Splenic lymphocyte cells |
| Concentration: | 5.5 μg/ml, 11 μg/ml, 22 μg/ml |
| Incubation Time: | 48 hours |
| Result: | Caused S stage cells and G2/M stage cells decreased. |
Enmein (inject to ear; 6.52-13.04 mg/kg; 5 days) inhibits the xylene-induced ear swelling and inhibits IL-2 expression.
| Animal Model: | Balb/c mice |
| Dosage: | 6.52 mg/kg, 9.78 mg/kg or 13.04 mg/kg |
| Administration: | Injected to ear; 6.52 mg/kg, 9.78 mg/kg or 13.04 mg/kg; 5 days |
| Result: | Had inhibitory effect on IL-2 release in blood. |
化学性质
白色针状色结晶，可溶于甲醇、乙醇、DMSO等有机溶剂，来源于溪黄草。