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| | Disodium 4-nitrophenylphosphate Basic information |
| | Disodium 4-nitrophenylphosphate Chemical Properties |
| Melting point | >300 °C(lit.) | | density | 1.751 at 20℃ | | vapor pressure | 15Pa at 20-50℃ | | storage temp. | -20°C | | solubility | H2O: 1 tablet/3 mL 25 mM, clear, light yellow | | form | tablets | | color | white to pale yellow | | Water Solubility | Miscible with water. Immiscible with ethanol and acetone. | | BRN | 5324661 | | Stability: | Stable, but may be light sensitive. Incompatible with strong acids, strong bases, strong oxidizing agents. | | InChIKey | KAKKHKRHCKCAGH-UHFFFAOYSA-L | | LogP | -2.16 at 22℃ and pH6.7 | | Surface tension | 69.34mN/m at 1.051g/L and 20℃ | | CAS DataBase Reference | 4264-83-9(CAS DataBase Reference) | | EPA Substance Registry System | Phosphoric acid, mono(4-nitrophenyl) ester, disodium salt (4264-83-9) |
| | Disodium 4-nitrophenylphosphate Usage And Synthesis |
| Chemical Properties | White crystalline | | Uses | PNPP, Disodium Salt is a colorimetric alkaline phosphatase soluble substrate, p-Nitrophenyl Phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in Enzyme Linked Immunosorbant Assay (ELISA) procedures. | | General Description | Excellent substrate for alkaline phosphatase-based ELISA assays. Produces a yellow soluble end product that can be read at 405 nm to 410 nm. Both kinetic and endpoint determinations can be performed. ε max: ≥9600 cm-1 M-1 at 311 nm in 0.4 M Na2CO3. | | Biochem/physiol Actions | Primary TargetSubstrate for phosphatase | | Purification Methods | Dissolve it in hot aqueous MeOH, filter and precipitate it by adding Me2CO. Wash the solid with Me2CO and repeat the purification. Aqueous MeOH and Et2O can also be used as solvents. The white fibrous crystals contain less than 1% of free 4-nitrophenol [assay: Axelrod J Biol Chem 167 57 1947]. [Beilstein 6 IV 1327.] |
| | Disodium 4-nitrophenylphosphate Preparation Products And Raw materials |
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