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| | Coumarin 151 Basic information |
| Product Name: | Coumarin 151 | | Synonyms: | 7-amino-4-(trifluoromethyl)-2-benzopyrone;Coumarin 490;7-AMINO-4-(TRIFLUOROMETHYL)COUMARIN, FOR FLUORESCENCE;7-AMIDO-4-TRIFLUOROMETHYLCOUMARIN;7-AMINO-4-(TRIFLUOROMETHYL)COUMARIN, 99+ %;Caspase 8 Fluorometric Substrate, IETD-AFC (FLICE, MASH, Mch5, N-acetyl-Asp-Glu-Val-Asp-AFC;Coumarin 151, laser grade, pure, 99%;7-Amino-4-(trifluoromethyl)coumarin 99% | | CAS: | 53518-15-3 | | MF: | C10H6F3NO2 | | MW: | 229.16 | | EINECS: | 258-599-1 | | Product Categories: | Scintillators;Coumarin | | Mol File: | 53518-15-3.mol |  |
| | Coumarin 151 Chemical Properties |
| Melting point | 221-222 °C(lit.) | | Boiling point | 314.6±42.0 °C(Predicted) | | density | 1.4096 (estimate) | | storage temp. | under inert gas (nitrogen or Argon) at 2–8 °C | | pka | 1.12±0.20(Predicted) | | form | Powder | | color | Yellow | | Water Solubility | Soluble in dimethyl sulfoxide (25 mg/ml), dimethyl formamide (25 mg/ml), and methanol (25 mg/ml). Insoluble in water. | | BRN | 4456797 | | CAS DataBase Reference | 53518-15-3(CAS DataBase Reference) | | EPA Substance Registry System | 2H-1-Benzopyran-2-one, 7-amino-4-(trifluoromethyl)- (53518-15-3) |
| | Coumarin 151 Usage And Synthesis |
| Chemical Properties | Coumarin 151 is yellow powder | | Uses | Coumarin 151 is used to prepare peptidase substrates and for the synthesis of substrates for the fluorometric assay of proteolytic enzymes in biological fluids. | | Uses | 7-Amino-4-(trifluoromethyl)coumarin is used in the synthesis of substrates for the fluorometric assay of proteolytic enzymes in biological fluids. It is suitable as laser dye. | | Definition | ChEBI: 7-amino-4-(trifluoromethyl)coumarin is a member of 7-aminocoumarins. It has a role as a fluorochrome. | | Purification Methods | Purify the coumarin by column chromatography on a C18 column, elute with acetonitrile/0.01M H2O/HCl (1:1), and recrystallise it from isopropanol. Alternatively, it is eluted from a silica gel column with CH2Cl2, or by extracting a CH2Cl2 solution (4g/L) with 1M aqueous NaOH (3 x 0.1L), followed by drying (MgSO4), filtration and evaporation. [Bissell J Org Chem 45 2283 1980, Zimmermann et al. Anal Biochem 70 258 1976.] |
| | Coumarin 151 Preparation Products And Raw materials |
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