香豆素 151
中文名称 | 香豆素 151 |
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中文同义词 | 7-氨基-4-三氟甲基香豆素;香豆素 151;7-氨基-4-(三氟甲基)-2H-苯并吡喃-2-酮;7-氨基-4-(三氟甲基)苯并吡喃-2-酮;2H-1-苯并吡喃-2-酮,7-氨基-4-(三氟甲基)-;7-氨基-4-三氟甲基香豆素,AMC151;7-氨基-4-三氟甲基香豆素,99%;香豆素490 |
英文名称 | Coumarin 151 |
英文同义词 | 7-amino-4-(trifluoromethyl)-2-benzopyrone;Coumarin 490;7-AMINO-4-(TRIFLUOROMETHYL)COUMARIN, FOR FLUORESCENCE;7-AMIDO-4-TRIFLUOROMETHYLCOUMARIN;7-AMINO-4-(TRIFLUOROMETHYL)COUMARIN, 99+ %;Caspase 8 Fluorometric Substrate, IETD-AFC (FLICE, MASH, Mch5, N-acetyl-Asp-Glu-Val-Asp-AFC;Coumarin 151, laser grade, pure, 99%;7-Amino-4-(trifluoromethyl)coumarin 99% |
CAS号 | 53518-15-3 |
分子式 | C10H6F3NO2 |
分子量 | 229.16 |
EINECS号 | 258-599-1 |
相关类别 | 香豆素;有机砌块;医药中间体;杂环砌块;标准品,对照品;对照品;Scintillators;Coumarin;化工 |
Mol文件 | 53518-15-3.mol |
结构式 |
香豆素 151 性质
熔点 | 221-222 °C(lit.) |
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沸点 | 314.6±42.0 °C(Predicted) |
密度 | 1.4096 (estimate) |
储存条件 | under inert gas (nitrogen or Argon) at 2–8 °C |
酸度系数(pKa) | 1.12±0.20(Predicted) |
形态 | 粉末 |
颜色 | 粘黄色 |
水溶解性 | Soluble in dimethyl sulfoxide (25 mg/ml), dimethyl formamide (25 mg/ml), and methanol (25 mg/ml). Insoluble in water. |
BRN | 4456797 |
CAS 数据库 | 53518-15-3(CAS DataBase Reference) |
EPA化学物质信息 | 2H-1-Benzopyran-2-one, 7-amino-4-(trifluoromethyl)- (53518-15-3) |
Amino acid and peptide derivatives of 7-amino-4-(trifluoromethyl)coumarin are used to monitor peptidase activities. Caspase activation is measured using the fluorogenic compound N-acetyl-asp-glu-val-asp-7-Amino-4-(trifluoromethyl)coumarin (Ac-DEVD-AFC). This substrate rapidly enters cells where it is efficiently cleaved at the aspartate residue by specific caspases, yielding the fluorescent compound 7-Amino-4-(trifluoromethyl)coumarin (AFC). Following cell disruption, released 7-Amino-4-(trifluoromethyl)coumarin is separated on HPLC and detected by fluorescence. The appearance of 7-Amino-4-(trifluoromethyl)coumarin in cells is blocked by the pancaspase inhibitor benzyloxycarbonyl-val-ala-asp-fluoromethylketone, thus establishing that intracellular caspases are responsible for the cleavage. γ-glutamyl-7-amino-4-(trifluoro-methyl)coumarin is synthesized and used as a substrate for fluirimetric assay of γ-gluta-myltranspeptidase. The reaction product 7-amino-4-(trifluoromethyl)coumarin is fluorescent at neutral pH values and with excitation and emission wavelengths of 400 and 490 nm, respectively, concentration is linearly related to fluorescence over the range of 10 to 300 pmol/3 mL reaction mixture.
安全信息
危险品标志 | Xi,Xn |
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危险类别码 | 36/37/38-20/21/22 |
安全说明 | 26-36-37/39 |
WGK Germany | 3 |
TSCA | T |
危险等级 | IRRITANT |
海关编码 | 29322090 |
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更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
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2024/08/19 | A18400 | 7-氨基-4-三氟甲基香豆素 7-Amino-4-(trifluoromethyl)coumarin | 53518-15-3 | 0.25g | 332元 |
2024/08/19 | A18400 | 7-氨基-4-三氟甲基香豆素, 99%
7-Amino-4-(trifluoromethyl)coumarin, 99% | 53518-15-3 | 1g | 982元 |