| Company Name: |
VDM Biochemicals
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0330-2528181 |
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sales@vdmbio.com |
| Products Intro: |
Product Name:Apoptosis Inhibitor II, NS3694
CAS:426834-38-0
Purity:>=95% by HPLC Package:80USD/10mg
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| | NS 3694 Basic information |
| Product Name: | NS 3694 | | Synonyms: | Apoptosis Inhibitor II, NS3694;Apoptosis Inhibitor II, NS3694 - CAS 426834-38-0 - Calbiochem;Benzoic acid, 4-chloro-2-[[[[3-(trifluoromethyl)phenyl]amino]carbonyl]amino]-;NS 3694,Inhibitor,Apaf-1,Apoptosis,inhibit,caspase-9,NS-3694,apoptosome inhibitor,NS3694,cytochrome c | | CAS: | 426834-38-0 | | MF: | C15H10ClF3N2O3 | | MW: | 358.7 | | EINECS: | | | Product Categories: | Apoptosis Inhibitors | | Mol File: | 426834-38-0.mol |  |
| | NS 3694 Chemical Properties |
| storage temp. | 2-8°C | | solubility | DMSO: 22 mg/mL, soluble | | form | solid |
| | NS 3694 Usage And Synthesis |
| Description | Cytochrome c, which leaks from the mitochondrial intermembrane space into the cytosol, causes oligomerization of apoptotic protease-activating factor 1 (Apaf-1), forming a caspase activation complex known as the apoptosome. NS3694 is a cell-permeable diarylurea compound that inhibits apoptosome formation and caspase activation. It blocks cytochrome c-induced caspase activation in HeLa cell cytosolic extracts with an IC50 value of approximately 50 μM. NS3694 prevents the formation of apoptosomes, which prevents the association of procaspases with Apaf-1. It suppresses caspase activation and apoptosis in cells treated with either TNF-α or staurosporine . NS3694 is used to examine the role of apoptosome-mediated caspase activation in cell death. | | Uses | NS3694 is a suppressor of the formation of apoptosome Apaf-1. | | Definition | ChEBI: 4-chloro-2-[[oxo-[3-(trifluoromethyl)anilino]methyl]amino]benzoic acid is a member of ureas. | | in vitro | previous study found that ns3694, and its two analogs (ns1764 and ns1784) were well-tolerated by mcf-7s1 breast cancer cells at concentrations up to 100, 50, and 25 μm, respectively. moreove, all three compounds could not inhibit recombinant caspase 9 and caspase 3 at concentrations ranging from 25 to 100 μm. in addition, ns3694 was able to inhibit the co-immunoprecipitation of caspase 9 and apaf-1 from hela cell cytosol stimulated by cytochrome c and datp. ns3694 could also inhibit the formation of the active 700-kda apoptosome complex, but had no effect on tnf-induced caspase-independent death of wehi-s cells. ns3694 did not inhibit fasl-induced caspase activation or death in type i cells neither [1]. | | IC 50 | approximately 50 μm for cytochrome c-induced caspase activation in hela cell cytosolic extracts | | references | [1] lademann, u. ,cain, k.,gyrd-hansen, m., et al. diarylurea compounds inhibit caspase activation by preventing the formation of the active 700-kilodalton apoptosome complex. mol.cell biol. 23(21), 7829-7837 (2003). |
| | NS 3694 Preparation Products And Raw materials |
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