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| | ALPHA-NAPHTHYL PHOSPHATE DISODIUM SALT Basic information |
| Product Name: | ALPHA-NAPHTHYL PHOSPHATE DISODIUM SALT | | Synonyms: | AURORA KA-1623;1-Naphthyl phsophoric acid sodium salt;α-Naphthyl phosphate disodium salt;disodium naphthyl phosphate;A-NAPHTHYL PHOSPHATE DISODIUM;1-NAPHTHYL PHOSPHATE DISODIUM SALT HYDRATE 99%;NAPHTHYLPHOSPHORIDE 2 NA SALT;PHOSPHORIC ACID 1-NAPHTHYL ESTER 2 NA SALT | | CAS: | 2183-17-7 | | MF: | C10H10NaO4P | | MW: | 248.15 | | EINECS: | 218-564-3 | | Product Categories: | | | Mol File: | 2183-17-7.mol |  |
| | ALPHA-NAPHTHYL PHOSPHATE DISODIUM SALT Chemical Properties |
| | ALPHA-NAPHTHYL PHOSPHATE DISODIUM SALT Usage And Synthesis |
| Uses | 1-Naphthyl phosphate disodium salt has been used:
- as a component in the mixture for soaking the chromatography paper for determining acid phosphatase activity
- in the staining of the alkaline phosphatase isoforms in 7.5% non-denaturing polyacrylamide gel
- as a component in the mixture for soaking the chromatography paper for preparing acid phosphomonoesterase-reactive imprinting sheets
- as a component in the staining solution for staining the roots for determining phosphomonoesterase activity
| | Purification Methods | Purify the salt through an acid ion-exchange column (in H+ form) to give the free acid [1136-89-6], M 224.2, which is obtained by freeze drying and recrystallising from Me2CO/*C6H6, or by adding 2.5volumes of hot CHCl3 (or 20 parts of boiling *C6H6) to a hot solution of 1 part acid and 1.2 parts Me2CO and cooling (m 155-157o, 157-158o). The acid is dissolved in the minimum volume of H2O to which 2 equivalents of NaOH are added and then freeze dried, or by adding the equivalent amount of MeONa in MeOH to a solution of the acid in MeOH and collecting the Na salt, washing with cold MeOH, then Et2O, and drying in a vacuum. [Friedman & Seligman J Am Chem Soc 72 624 1950, Chanley & Feageson J Am Chem Soc 77 4002 1955.] The monosodium salt [1136-89-6] is similarly prepared but by using 1 equivalent of NaOH. The phosphate group is hydrolysed at pH 1.1-5.85 at 70o. [Beilstein 6 IV 4226.] It is a substrate for alkaline phosphatase [Gomori Methods Enzymol 4 381 1957, 128 212 1968], and prostatic phosphatase [Babson Clin Chem 30 1418 1984]. [See p 538 in “Metal-organic Compounds”, Chapter 5, Beilstein 6 IV 4226.] |
| | ALPHA-NAPHTHYL PHOSPHATE DISODIUM SALT Preparation Products And Raw materials |
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