L-4-Fluorophenylalanine synthesis
- Product Name:L-4-Fluorophenylalanine
- CAS Number:1132-68-9
- Molecular formula:C9H10FNO2
- Molecular Weight:183.18
459-32-5
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1132-68-9
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$12.50/1G
Yield:-
Reaction Conditions:
with phenylalanine ammonia lyase from Rhodotorula graminis;ammonium carbamate in aq. buffer; pH=9 at 30;Kinetics;Reagent/catalyst;
Steps:
4.6.3 Kinetics in the amination reaction (Assay 2)
General procedure: Solutions of the substrates under investigation were made up in 0.5M ammonium carbamate/1M Tris, pH 9.0 and dilutions were made with the following concentrations: 3, 2, 1, 0.5, 0.2, 0.02, 0.002 and 0mM of cinnamic acid. Scopoletin solution - 288.25mg of scopoletin was dissolved in 50mL dH2O to make a stock solution. This stock solution was then diluted 1/5 to create a working solution that had a fluorescence of approximately 30,000 RFU. The screening was set up in a 96-well plate and each well contained the following: 100μL substrate solution, 10μL HRP (1mg/mL in dH2O), 10μL l-AAO (1 in 10 dilution of stock in dH2O), 40μL scopoletin solution, 40μL purified PAL. Kinetic data was measured on a plate reader at 30°C. Excitation wavelength 360nm; emission wavelength 480nm; 15s interval.
References:
Rowles, Ian;Groenendaal, Bas;Binay, Baris;Malone, Kirk J.;Willies, Simon C.;Turner, Nicholas J. [Tetrahedron,2016,vol. 72,# 46,p. 7343 - 7347]
7761-30-0
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3060-50-2
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119-61-9
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1132-68-9
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18125-46-7
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51-65-0
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1132-68-9
261 suppliers
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18125-46-7
211 suppliers
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7761-30-0
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1132-68-9
261 suppliers
$12.50/1G
207910-84-7
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1132-68-9
261 suppliers
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