| Identification | Back Directory | [Name]
Calcein tetraethyl ester | [CAS]
1170856-93-5 | [Synonyms]
Calcein tetraethyl ester
Fluorexon tetraethyl ester
Calceintetraethylester, >97%
Tetraethyl Fluorescein Bis(methylene)bis(azanetriyl)tetraacetate
Tetraethyl 2,2',2'',2'''-(((3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-4',5'-diyl)bis(methylene))bis(azanetriyl))tetraacetate
N,N'-[(3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-4',5'-diyl)bis(methylene)]bis[N-(2-ethoxy-2-oxoethyl)-glycine] 1,1'-diethyl ester
Glycine, N,N'-[(3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-4',5'-diyl)bis(methylene)]bis[N-(2-ethoxy-2-oxoethyl)-, 1,1'-diethyl ester | [Molecular Formula]
C38H42N2O13 | [MDL Number]
MFCD14586332 | [MOL File]
1170856-93-5.mol | [Molecular Weight]
734.75 |
| Chemical Properties | Back Directory | [Melting point ]
146 - 151°C | [Boiling point ]
825.4±65.0 °C(Predicted) | [density ]
1.41±0.1 g/cm3(Predicted) | [storage temp. ]
-20°C Freezer, Under inert atmosphere | [solubility ]
Acetonitrile (Slightly), Methanol (Slightly, Heated) | [form ]
Solid | [pka]
9.20±0.20(Predicted) | [color ]
Off-White to Light Yellow |
| Hazard Information | Back Directory | [Uses]
Tetraethyl Fluorescein Bis(methylene)bis(azanetriyl)tetraacetate is an intermediate used in the synthesis of Calcein AM (C125400), which is a hydrophobic non-fluorescent probe that can permeate the plasma membrane and can be hydrolyzed to Calcein (sc-202090), which is an extremely fluorescent and negatively charged molecule. Calcein-AM can be used to stain living cells and in cytoplasmic Schwann cells has been observed to fluoresce bright green. Calcein-AM has been reported to be a neutral substrate for P-glycoprotein (Pgp) and multidrug resistance protein (MRP) and has been used in flow cytometry studies to analyze the function of P-gp and MRP. |
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