| Chemical Properties | Back Directory | [storage temp. ]
-20°C | [solubility ]
Soluble in DMSO, DMF, DCM | [form ]
powder | [color ]
Brown to black | [Appearance]
Dark blue solid | [ex/em]
640/664 nm (PBS buffer) | [ε(extinction coefficient)]
250000 L⋅mol−1⋅cm−1 | [Φ(quantum yield)]
0.2 | [InChIKey]
VOWXUNAERRNFLE-UHFFFAOYSA-N | [SMILES]
C([N+]1=C(C(C)(C)C2=CC=CC=C12)C=CC=CC=C1C(C2=CC=CC=C2N1C)(C)C)CCCCC(=O)NCC#C.[Cl-] |
| Safety Data | Back Directory | [Hazard Codes ]
Xi | [Risk Statements ]
36/37/38 | [Safety Statements ]
26 | [WGK Germany ]
WGK 3 | [Storage Class]
11 - Combustible Solids | [Hazard Classifications]
Eye Irrit. 2
Skin Irrit. 2
STOT SE 3 |
| Hazard Information | Back Directory | [Description]
Cy5 alkyne is a cynaine linker containing an alkyne group, which enables Click Chemistry to attach deeply colored and photostable Cyanine5 fluorophore to various azide-bearing molecules. Cyanine5 is a popular fluorophore that has been widely used for various applications including intact organism imaging. | [Chemical Properties]
Appearance: Dark blue solid
ex/em: 640/664 nm (PBS buffer)
Extinction coefficient (ε): 250000 L??mol??1??cm??1
Quantum yield (Φ): 0.2 | [Uses]
Cyanine5 alkyne (Alkyne-Cy5) is a fluorescent dye used to label azide proteins and can be used to analyse post-translational modifications of proteins, glycosylation etc. Cyanine5 alkyne can also be used as a mitochondrial OXPHOS inhibitor to inhibit the growth of cancer stem cells (CSC)[1][2]. Cyanine5 alkyne is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups. | [References]
[1] Amanda R Burnham-Marusich, et al. Size-matched alkyne-conjugated cyanine fluorophores to identify differences in protein glycosylation. Electrophoresis. 2014 Sep;35(18):2621-5. DOI:10.1002/elps.201400241
[2] Camillo Sargiacomo, et al. MitoTracker Deep Red (MTDR) Is a Metabolic Inhibitor for Targeting Mitochondria and Eradicating Cancer Stem Cells (CSCs), With Anti-Tumor and Anti-Metastatic Activity In Vivo. Front Oncol. 2021 Jul 30;11:678343. DOI:10.3389/fonc.2021.678343 | [Abs/Em Maxima]
646/662 nm | [Fluoresene quantum yield]
0.2 | [Extinction Coefficient]
250000 | [CF260]
0.03 | [CF280]
0.04 | [Microscopy Laser Line]
633 or 635 nm | [Flow Cytometry Laser Line]
633 or 635 nm | [Spectrally Similar Dyes]
Alexa Fluor® 647, CF™ 647 Dye, DyLight™649 |
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| Company Name: |
Alfa Chemistry
|
| Tel: |
1-516-6625404 |
| Website: |
https://www.alfa-chemistry.com |
| Company Name: |
Henan Alfachem Co.,Ltd.
|
| Tel: |
0371-55051623 18137891487 |
| Website: |
www.chemicalbook.com/supplier/14555231/ |
|