| Identification | Back Directory | [Name]
PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1 | [CAS]
136112-00-0 | [Synonyms]
PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1
PHENOL:CHLOROFORM:ISOAMYLALCOHOL 49.5:49 .5:1
phenol - chloroform - isoamyl alcohol mixture
PHENOL/CHLOROFORM/ISOAMYL ALCOHOL (25:24:1), STABILZED,
Phenol/Chloroform/Isoamyl alcohol (25:24:1), stabilized
PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1, FOR MOL. BIOLOGY
Phenol mixt. with 3-methyl-1-butanol and trichloromethane
PHENOL:CHLOROFORM:ISOAMYL ALC. 125:24:1, FOR MOL. BIOLOGY
Phenol/Chloroform/Isoamyl alcohol (25:24:1),stabilized,for molecular biology,DNAse, RNAse
Phenol/Chloroform/Isoamyl alcohol (25:24:1)stabilzedDNAseRNAse and Protease freepH 7.8-8.2for molecular biology
Phenol/Chloroform/Isoamyl alcohol (25:24:1), stabilized, DNAse, RNAse and Protease free, pH 7.8-8.2, for molecular biology
Phenol/ChloroforM/IsoaMyl alcohol (25:24:1), stabilized, for Molecular biology, DNAse, RNAse and Protease free, pH 7.8-8.2 | [Molecular Formula]
C6H6O.C5H12O.CHCl3 | [MDL Number]
MFCD00133763 | [MOL File]
136112-00-0.mol | [Molecular Weight]
301.637 |
| Chemical Properties | Back Directory | [Appearance]
clear yellow solution | [density ]
1.29 g/mL at 20 °C
| [Fp ]
79 °C | [storage temp. ]
2-8°C
| [form ]
Liquid | [PH]
7.7-8.3(H2O-phase, after extraction with H2O, 1:1) | [Water Solubility ]
Soluble in water. | [λmax]
λ: 330 nm Amax: 0.2
λ: 405 nm Amax: 0.1
λ: 510 nm Amax: 0.1 | [InChI]
InChI=1S/C6H6O.C5H12O.CHCl3/c7-6-4-2-1-3-5-6;1-5(2)3-4-6;2-1(3)4/h1-5,7H;5-6H,3-4H2,1-2H3;1H | [InChIKey]
ZYWFEOZQIUMEGL-UHFFFAOYSA-N | [SMILES]
ClC([H])(Cl)Cl.O([H])C([H])([H])C([H])([H])C([H])(C([H])([H])[H])C([H])([H])[H].O([H])C1C([H])=C([H])C([H])=C([H])C=1[H] | [Absorption]
cut-off at 360nm |
| Hazard Information | Back Directory | [Chemical Properties]
clear yellow solution | [Uses]
Phenol:Chloroform:Isoamyl alcohol 25:24:1 is used in the extraction of DNA for the generation of DNA minisatellite fingerprints. It is also used for purifying RNA. | [Biological Activity]
When nucleic acids are extracted with the Phenol-Chloroform-Isoamyl alcohol mixture solutiona clear component separation is achieved. The proteins present in the sample are denatured and removed (collected in the organic phase or in the interphase)while nucleic acids remain in the aqueous phase. The chloroform denatures proteins and facilitates the separation of the aqueous and organic phasesand the isoamyl alcohol reduces foaming during extraction. |
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| Company Name: |
chemmole Gold
|
| Tel: |
400-168-9330 15013243073 |
| Website: |
http://www.chemmolemall.com/ |
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