| Identification | Back Directory | [Name]
GRANZYME B | [CAS]
143180-74-9 | [Synonyms]
GZMB
GRANZYME B
EC 3.4.21.79
GRANZYME B ENZYME
GRANZYME B (HUMAN)
Granzyme B from mouse
Granzyme B from Human, Recombinant
Granzyme B from Mouse, Recombinant
Recombinant Mouse Gzmb Protein, His Tag
GRANZYME B, HUMAN, CELL CULTURE-DERIVED
SensiZyme Granzyme B Activity Assay Kit | [MDL Number]
MFCD01323415 |
| Chemical Properties | Back Directory | [storage temp. ]
-70°C | [form ]
buffered aqueous solution | [color ]
white to off-white | [biological source]
mouse | [Specific Activity]
≥40units/μg |
| Hazard Information | Back Directory | [Uses]
Granzyme B is a cysteine protease found in the cytoplasmic granules of cytolytic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme B is required for the induction of target cell lysis, which occurs as part of cell mediated immune responses, and can activate apoptosis in target cells by both caspase dependent and caspase independent mechanisms. Proteolytic cleavage of substrates by Granzyme B takes place primarily after aspartic acid residues. Recombinant murine Granzyme B is a glycosylated 227 amino acid protein, comprising the mature active portion of the murine Granzyme B precursor. The apparent molecular weight is 28.9 kDa by mass spectrometry. | [General Description]
Granzyme B, a 247 amino acid polypeptide, contains a leader sequence, which is cleaved by a signal peptidase and a two amino acid prodomain, which is cleaved by the lysosomal cysteine protease DPPI. The recombinant granzyme B is expressed in Pichia pastoris as the mature form and appears on SDS-PAGE as a triplet (~34, 32, and 30 kDa) due to three different glycosylations. | [Biochem/physiol Actions]
Cytotoxic granules secreted by natural killer (NK) cells and cytotoxic T Lymphocytes cells are part of the mechanism used for protecting the organism from virus infected and tumor cells. Granzyme B, a serine protease, is the most prominent granzyme in a family of 11 found in the cytotoxic granules. The granzymes enter the target cell with the assistance of perforin, a critical molecule of the cytotoxic granules. In the target cell, the granzymes act on specific substrates involved with the cell death via apoptosis.Granzyme B is a neutral serine protease that cleaves aspartic acid residues, inducing cell death by various pathways. It can cleave and activate most of the caspases in vitro and in vivo resulting in a massive amplification of the caspase dependent apoptotic pathway. In addition, granzyme B cleaves directly downstream caspase substrates as PARP DNA-PK2 and DFF45/ICAD leading to cell death. This pathway bypasses inhibition of apoptosis by viral caspase inhibitors found in virus infected cells. It has been shown that granzyme B is capable of inducing cytochrome C release from the mitochondria in a caspase independent way. |
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Biolab Reagents
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Merck KGaA
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shlmai
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Creative Enzymes
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