| Identification | Back Directory | [Name]
DOTAP | [CAS]
144189-73-1 | [Synonyms]
DOTAP
DOTAP METHOSULFATE
DOTAP methyl sulfate
TRANSFECTION REAGENT
18:1 TAP (DOTAP, MS Salt)
DOTAP Transfection Reagent
TRANSFAST(TM) TRANSFECTION REAGENT
TFX(TM) REAGENTS TRANSFECTION TRIO
DOTAP Liposomal Transfection Reagent
N-(2,3-dioleoyloxy-1-propyl)trime.-amm. me. sulfate
N-(2,3-DIOLEOYLOXY-1-PROPYL)TRIME.-AMM. ME. SULFATE,5X0.4ML
N-(2,3-DIOLEOYLOXY-1-PROPYL)TRIMETHYLAMMONIUM METHYL SULFATE
N-[1-(2,3-DIOLEOYLOXY)PROPYL]-N,N,N-TRIMETHYLAMMONIUM METHYL-SULFATE
DOTAP Liposomal Transfection Reagent N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate | [Molecular Formula]
C43H83NO7S | [MDL Number]
MFCD16660441 | [MOL File]
144189-73-1.mol | [Molecular Weight]
758.19 |
| Chemical Properties | Back Directory | [density ]
1.11 g/cm3 | [storage temp. ]
2-8°C | [solubility ]
Acetonitrile: Slightly soluble
Water: Slightly soluble | [form ]
buffered aqueous suspension | [color ]
clear to hazy colorless to faint yellow | [InChIKey]
RSMRWWHFJMENJH-LQDDAWAPSA-M | [SMILES]
C(C[N+](C)(C)C)(COC(=O)CCCCCCC/C=C\CCCCCCCC)OC(=O)CCCCCCC/C=C\CCCCCCCC.S([O-])(=O)(=O)OC |
| Hazard Information | Back Directory | [Description]
18:1 TAP (DOTAP, MS Salt) is a cationic derivative of trimethylammonium linked with two 18-carbon fatty acid tails with single alkene groups in each tail. 18:1 TAP is a cationic liposome-forming compound that may be used for transfection of DNA, RNA, and other negatively charged molecules into eukaryotic cells. | [Uses]
The DOTAP (N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate) Liposomal Transfection Reagent is a liposome formulation of the cationic lipid DOTAP. DOTAP can be used for the highly efficient transfection of DNA including yeast artificial chromosomes (YACs) into eukaryotic cells for transient or stable gene expression, and is also suitable for the efficient transfer of other negatively charged molecules, such as RNA, oligonucleotides, nucleotides, ribonucleo-protein (RNP) complexes, and proteins into research samples of mammalian cells. | [General Description]
DOTAP Liposomal Transfection Reagent is a liposome formulation of the cationic lipid DOTAP. Unlike other liposomal reagents, it produces highly efficient transfections both in the presence and absence of serum. It can also be successfully used for in vivo applications. Mixing DOTAP with DNA results in spontaneously formed stable complexes. These complexes can be added directly to the cell culture medium. This method of DNA transfer is very gentle, avoiding the cytotoxic effects commonly encountered with lipofection or other transfection methods. | [Biochem/physiol Actions]
DOTAP (1,2-dioleoyl-3-trimethylammonium-propane) is a cationic lipid, used for in vitro and in vivo nucleic acid and protein delivery. The lipid composition and cell type affects the transfection potential of DOTAP. DOTAP mediates gene knockdown technique in hematopoietic stem cells (HSCs). | [References]
[1] F XIONG N G Z Mi. Cationic liposomes as gene delivery system: transfection efficiency and new application.[J]. Pharmazie, 2011, 66 3: 158-164.
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| Company Name: |
J & K SCIENTIFIC LTD.
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| Telephone: |
18210857532 18210857532 |
| Website: |
https://www.jkchemical.com |
| Company Name: |
Sigma-Aldrich
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| Telephone: |
021-61415566 800-8193336 |
| Website: |
https://www.sigmaaldrich.cn |
| Company Name: |
Rhawn Reagent
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| Telephone: |
400-400-1332688 18019345275 |
| Website: |
http://www.rhawn.cn |
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