ChemicalBook--->CAS DataBase List--->1454334-76-9

1454334-76-9

1454334-76-9 Structure

1454334-76-9 Structure
IdentificationBack Directory
[Name]

6-Azido-L-norleucine HCl
[CAS]

1454334-76-9
[Synonyms]

H-Lys(N3)-OH.HCl
6-Azido-L-norleucine HCl
L-Azidonorleucine hydrochloride
Nε-Azido-L-Lysine hydrochloride
(2S)-2-amino-6-azidohexanoic acid
(S)-2-Amino-6-azidohexanoic acid hydrochloride
(2S)-2-amino-6-azidohexanoic acid hydrochloride
[Molecular Formula]

C6H13ClN4O2
[MDL Number]

MFCD18382040
[MOL File]

1454334-76-9.mol
[Molecular Weight]

208.646
Chemical PropertiesBack Directory
[storage temp. ]

Store at -20°C
[solubility ]

Soluble in DMSO
[form ]

Solid
[color ]

White to off-white
[Water Solubility ]

Soluble to 100 mM in water
Safety DataBack Directory
[Symbol(GHS) ]

Exclamation Mark (GHS07)
GHS07
[Signal word ]

Warning
[Hazard statements ]

H319-H315-H335
[Precautionary statements ]

P264-P280-P305+P351+P338-P337+P313P-P264-P280-P302+P352-P321-P332+P313-P362
Hazard InformationBack Directory
[Uses]

L-Azidonorleucine hydrochloride, an unnatural amino acid, is A Methionine surrogate. L-Azidonorleucine hydrochloride can be used to label mammalian cell proteins and identify a diverse set of methionyl-tRNA synthetase (MetRS) mutants [1][2]. L-Azidonorleucine (hydrochloride) is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
[in vivo]

When L-Azidonorleucine (ANL) is administered via the drinking water, an average daily L-Azidonorleucine intake of 0.9 mg/day/g of body weight for 21 d when aiming to label and identify neuronal populations of the brain are recommended. Lower dosages or shorter labeling times might be used for the labeling of other cell types. When L-Azidonorleucine is administered by i.p. injection, the testing different L-Azidonorleucine doses in the range of 4-400 mM are recommended. A 400 mM dosage administered by i.p. (i.p. volume: 10 ml/kg) once per day for 1 week is recommended for labeling some neuronal populations (such as excitatory neurons of the hippocampus). Lower dosages (e.g., 4 mM) can potentially be used for labeling other cell types or when longer labeling periods are desired[2].

[storage]

Store at -20°C
[References]

[1] Alborz Mahdavi, et al. Engineered Aminoacyl-tRNA Synthetase for Cell-Selective Analysis of Mammalian Protein Synthesis. J Am Chem Soc. 2016 Apr 6;138(13):4278-81. DOI:10.1021/jacs.5b08980
[2] Beatriz Alvarez-Castelao, et al. Cell-type-specific metabolic labeling, detection and identification of nascent proteomes in vivo. Nat Protoc. 2019 Feb;14(2):556-575. DOI:10.1038/s41596-018-0106-6
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