| Identification | Back Directory | [Name]
EC 4.2.2.8 | [CAS]
149371-12-0 | [Synonyms]
EC 4.2.2.7
EC 4.2.2.8
HEPARITINASE
HEPARINASE II
HEPARINASE III
HEPARITINASE I
HEPARIN LYASE II
Heparin lyasr II
HEPARIN LYASE III
Lyase, heparin, II
rHeparinase Ⅱ (rHep Ⅱ)
HEPARAN SULFATE LYASE I
EC 4.2.2.8(HEPARIN LYASE II)
heparinase ii from flavobacterium*heparinum
Native Flavobacterium heparinum Heparinase II
Heparinase II from Bacteroides eggerthii, Recombinant
Heparinase II from Flavobacterium heparinum, Recombinant
Heparinase II from Flavobacterium heparinum,Heparin lyase II
Heparinase II from Flavobacterium heparinum Lyophilized powder stabilized with approx. 25% bovine serum albumin, lyophilized powder, >=100 units/mg protein (enzyme + BSA) | [Molecular Formula]
NULL | [MDL Number]
MFCD00131319 |
| Chemical Properties | Back Directory | [storage temp. ]
-20°C | [form ]
lyophilized powder | [biological source]
bacterial (Flavobacterium heparinum) | [Specific Activity]
≥100units/mg protein (enzyme + BSA) |
| Hazard Information | Back Directory | [Uses]
Heparinase II from Flavobacterium heparinum has been used:
- for digestion of heparin sulfate during exosome isolation
- for digestion of heparin sulfate in notochordal cell conditioned media (NCCM) to investigate the content of glycosaminoglycans in NCCM
- as a component of digestion buffer during cell surface glycan processing
- as a component of heparin lyase (HSase) mix to remove the heparin sulfate on the 293ͰT/ACE2 cell surface and study the effect of histones on the infectivity of pseudovirus
| [Biological Activity]
Heparinase II cleaves heparan sulfateand to a lesser extent heparin (relative activity about 2:1) at the α (1-4) linkages between hexosamines and uronic acid residues (both glucuronic and iduronic)yielding mainly disaccharides. Heparinase II has the broadest substrate specificity of the three heparinases Heparinase enzymes helps in decoding the complex structures of substrates. Heparin interferes with DNA transcription in PCR and in reverse transcription of RNA. Heparinase II has been used to remove heparin for downstream analysis of genomic DNA. |
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Sigma-Aldrich
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021-61415566 800-8193336 |
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https://www.sigmaaldrich.cn |
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