| Identification | Back Directory | [Name]
Z-Vad-fmk, non-methylated | [CAS]
161401-82-7 | [Synonyms]
Z-VAD-FMK, Free Acid
Z-VAD-FMK (Caspase Inhibitor VI)
Z-VAD(OH)-FMK (Caspase Inhibitor VI)
Z-VAL-ALA-ASP-(OH)-FLUOROMETHYL KETONE
Caspase Inhibitor VI - CAS 161401-82-7 - Calbiochem
Carbobenzyloxy-Val-Ala-Asp-alpha-fluoromethylketone
L-Alaninamide, N-[(phenylmethoxy)carbonyl]-L-valyl-N-[(1S)-1-(carboxymethyl)-3-fluoro-2-oxopropyl]-
L-Alaninamide, N-[(phenylmethoxy)carbonyl]-L-valyl-N-[1-(carboxymethyl)-3-fluoro-2-oxopropyl]-, (S)-
L-Alaninamide, N-[(phenylmethoxy)carbonyl]-L-valyl-N-[(1S)-1-(carboxymethyl)-3-fluoro-2-oxopropyl]- (9ci) | [Molecular Formula]
C21H28FN3O7 | [MOL File]
161401-82-7.mol | [Molecular Weight]
453.46 |
| Chemical Properties | Back Directory | [Boiling point ]
758.0±60.0 °C(Predicted) | [density ]
1.260±0.06 g/cm3(Predicted) | [storage temp. ]
-20°C | [solubility ]
DMSO:5.0(Max Conc. mg/mL);11.0(Max Conc. mM) | [form ]
Off-white solid | [pka]
3.91±0.19(Predicted) | [color ]
White to light yellow | [InChIKey]
KCZVXULVWPSIGV-YEWWUXTCSA-N | [SMILES]
C(N)(=O)[C@H](C)N(C(=O)[C@H](C(C)C)NC(OCC1=CC=CC=C1)=O)[C@@H](CC(O)=O)C(=O)CF |
| Hazard Information | Back Directory | [Description]
Z-VAD(OH)-FMK is an irreversible tripeptide inhibitor of all caspases. This non-methylated form is useful in studies involving recombinant, isolated, or purified enzymes. Unlike the methylated form, Z-VAD(OMe)-FMK , this compound does not require pretreatment with esterases prior to in vitro use. | [Uses]
Z-VAD-FMK (Z-VAD(OH)-FMK) is a well-know pan caspase inhibitor, which does not inhibit ubiquitin carboxy-terminal hydrolase L1 (UCHL1) activity even at concentrations as high as 440 μM[1]. | [General Description]
An irreversible general caspase inhibitor. Useful for studies involving recombinant, isolated, and purified caspase enzymes. Unlike Caspase Inhibitor I (Cat. No. 627610), this inhibitor does not require pretreatment with esterase for in vitro studies. A 10 mM (1 mg/221 μl) solution of Caspase Inhibitor VI (Cat. No. 219011) in DMSO is also available. | [Biochem/physiol Actions]
Product does not compete with ATP. | [IC 50]
Caspase | [References]
[1] Davies CW, et al. The co-crystal structure of ubiquitin carboxy-terminal hydrolase L1 (UCHL1) with a tripeptide fluoromethyl ketone (Z-VAE(OMe)-FMK). Bioorg Med Chem Lett. 2012 Jun 15;22(12):3900-4. DOI:10.1016/j.bmcl.2012.04.124
[2] Liu HR, et al. Antiproliferative activity of the total saponin of Solanum lyratum Thunb in Hela cells by inducing apoptosis. Pharmazie. 2008 Nov;63(11):836-42. PMID:19069247 |
|
|