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2129651-79-0

2129651-79-0 Structure

2129651-79-0 Structure
IdentificationBack Directory
[Name]

1,4-Benzenedicarboxylic acid, 2,5-dichloro-3-(2,4,5,7-tetrachloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)-, 1-(2,5-dioxo-1-pyrrolidinyl) ester
[CAS]

2129651-79-0
[Synonyms]

5-HEX-SE
HEX809 SE
5-HEX-NHS
1,4-Benzenedicarboxylic acid, 2,5-dichloro-3-(2,4,5,7-tetrachloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)-, 1-(2,5-dioxo-1-pyrrolidinyl) ester
[Molecular Formula]

C25H9Cl6NO9
[MOL File]

2129651-79-0.mol
[Molecular Weight]

680.06
Chemical PropertiesBack Directory
[Boiling point ]

861.5±75.0 °C(Predicted)
[density ]

1.96±0.1 g/cm3(Predicted)
[storage temp. ]

-20°C, stored under nitrogen
[solubility ]

very soluble in DMSO, DMF
[pka]

1.28±0.50(Predicted)
[Appearance]

White to off-white Solid
[InChIKey]

WRMUHYKDIQULCR-UHFFFAOYSA-N
[SMILES]

C1(C(ON2C(=O)CCC2=O)=O)=CC(Cl)=C(C(O)=O)C(C2C3=C(C(Cl)=C(O)C(Cl)=C3)OC3C=2C=C(Cl)C(=O)C=3Cl)=C1Cl
Hazard InformationBack Directory
[Description]

Activated NHS-ester of HEX (Hexachlorofluorescein) dye, a pure 6-isomer. This NHS ester is an amine-reactive dye for labeling of amine groups in proteins, peptides, amino-modified oligos, and other target molecules.


HEX is a hexachlorinated derivate of the fluorescent dye fluorescein. HEX exhibits an excitation peak at 533 nm and an emission peak at 549 in the green-yellow area. It can serve as a substitute for JOE, BODIPY™ 530/550, VIC™ because of their similar spectral characteristics.


HEX NHS ester is widely used in nucleic acid sequencing, PCR/qPCR, and related research.

[Abs/Em Maxima]

533/549 nm
[Fluoresene quantum yield]

0.57
[Extinction Coefficient]

87770
[CF260]

0.3
[CF280]

0.13
Spectrum DetailBack Directory
[Spectrum Detail]

1,4-Benzenedicarboxylic acid, 2,5-dichloro-3-(2,4,5,7-tetrachloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)-, 1-(2,5-dioxo-1-pyrrolidinyl) ester(2129651-79-0)1HNMR
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