| Identification | Back Directory | [Name]
H-MET-ALA-GLY-PRO-HIS-PRO-VAL-ILE-VAL-ILE-THR-GLY-PRO-HIS-GLU-GLU-OH | [CAS]
249537-73-3 | [Synonyms]
VIVIT
NFAT INHIBITOR
MAGPHPVIVITGPHEE
NFAT INHIBITOR?, >98%
NFAT Inhibitor (VIVIT peptide)
NFAT Inhibitor >96% (HPLC), solid
NFAT Inhibitor,VIVIT peptide,MAGPHPVIVITGPHEE?, >98%
MET-ALA-GLY-PRO-HIS-PRO-VAL-ILE-VAL-ILE-THR-GLY-PRO-HIS-GLU-GLU
H-MET-ALA-GLY-PRO-HIS-PRO-VAL-ILE-VAL-ILE-THR-GLY-PRO-HIS-GLU-GLU-OH
L-Glutamic acid, L-methionyl-L-alanylglycyl-L-prolyl-L-histidyl-L-prolyl-L-valyl-L-isoleucyl-L-valyl-L-isoleucyl-L-threonylglycyl-L-prolyl-L-histidyl-L-α-glutamyl- | [Molecular Formula]
C75H118N20O22S | [MDL Number]
MFCD02683959 | [MOL File]
249537-73-3.mol | [Molecular Weight]
1683.93 |
| Chemical Properties | Back Directory | [Boiling point ]
1982.4±65.0 °C(Predicted) | [density ]
1.334±0.06 g/cm3(Predicted) | [storage temp. ]
-20°C | [solubility ]
DMSO: 15 mg/ml; Water: 10 mg/ml | [form ]
solid
| [pka]
3.12±0.10(Predicted) | [color ]
White to off-white | [Water Solubility ]
Soluble to 2 mg/ml in water | [Sequence]
H-Met-Ala-Gly-Pro-His-Pro-Val-Ile-Val-Ile-Thr-Gly-Pro-His-Glu-Glu-OH |
| Hazard Information | Back Directory | [Description]
NFAT inhibitor is a cell-permeable compound that selectively inhibits calcineurin-mediated dephosphorylation of NFAT. At 100 μM, this inhibitor effectively blocks calcineurin binding to NFAT without disrupting other calcineurin-dependent pathways, unlike the immunosuppressants cyclosporin A and FK506 which indiscriminately block all calcineurin phosphatase activity. NFAT inhibitor can disrupt NFAT-dependent expression of IL-2 and TNF-α when transfected in Jurak T cells and can prevent the activation and proliferation of T cells both in vitro (~ 43% at 1 μM using mixed lymphocyte cultures) and in vivo (10 mg/kg using C3H/HeN mice). | [Uses]
NFAT Inhibitor, VIVIT has been used to study the signaling pathways involved in controlling inhibitory molecules and subsequent onset of T-cell impairment. | [Biochem/physiol Actions]
High-affinity calcineurin-binding peptide that inhibits NFAT (Nuclear Factor of Activated T cells) activation and NFAT-dependent expression of endogenous cytokine genes in T cells. | [storage]
Store at -20°C | [References]
[1] JOSE? ARAMBURU. Affinity-Driven Peptide Selection of an NFAT Inhibitor More Selective Than Cyclosporin A[J]. Science, 1999, 285 5436. DOI: 10.1126/science.285.5436.2129
[2] MICHAEL H A ROEHRL. Selective inhibition of calcineurin-NFAT signaling by blocking protein-protein interaction with small organic molecules.[J]. Proceedings of the National Academy of Sciences of the United States of America, 2004, 101 20: 7554-7559. DOI: 10.1073/pnas.0401835101
[3] HAIXIANG YU. Therapeutic potential of a synthetic peptide inhibitor of nuclear factor of activated T cells as antirestenotic agent.[J]. Arteriosclerosis, Thrombosis, and Vascular Biology, 2006, 26 7: 1531-1537. DOI: 10.1161/01.atv.0000225286.30710.af
[4] XUEQIANG ZHAO. JNK1 negatively controls antifungal innate immunity by suppressing CD23 expression[J]. Nature Medicine, 2017, 23 3: 337-346. DOI: 10.1038/nm.4260 |
|
|