| Identification | Back Directory | [Name]
DAF-FM DA | [CAS]
254109-22-3 | [Synonyms]
7′
9′
DAF-FM DA
DAF-FM DA solution
-[9H]xanthen]-3-one
-bis(acetyloxy)-2′
DAF-FM DA (cell permeable)
DAF-FM DA [DAF-FM Diacetate]
-difluoro-spiro[isobenzofuran-1(3H)
Diaminofluorescein-FM diacetate
DAF-FM DA - CAS 254109-22-3 - Calbiochem
4-Amino-5-(N-methylamin)-3′,6′-bis(acetyloxy)
3-Amino-4-(N-methylamino)-2', 7'-difluorofluorescein diacetate
4-Amino-5-(N-methylamino)-3′
4-Amino-2',7'-Difluoro-5-(Methylamino)-3-Oxo-3H-Spiro[2-Benzofuran-1,9'-Xanthene]-3',6'-Diyl Diacetate
Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-bis(acetyloxy)-4-amino-2',7'-difluoro-5-(methylamino)-
4-Amino-5-(N-methylamino)-3′,6′-bis(acetyloxy)-2′,7′-difluoro-spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one
DAF-FM DA;4-AMINO-5-(N-METHYLAMINO)-3′;6′-BIS(ACETYLOXY)-2′;7′-DIFLUORO-SPIRO[ISOBENZOFURAN-1(3H);9′-[9H]XANTHEN]-3-ONE;DIAMINOFLUORESCEIN-FM DIACETATE | [Molecular Formula]
C25H18F2N2O7 | [MDL Number]
MFCD16872050 | [MOL File]
254109-22-3.mol | [Molecular Weight]
496.42 |
| Chemical Properties | Back Directory | [Boiling point ]
695.7±55.0 °C(Predicted) | [density ]
1.56±0.1 g/cm3(Predicted) | [storage temp. ]
-20°C | [solubility ]
Soluble in dimethyl sulfoxide | [form ]
Pale yellow solution | [pka]
4.20 ± 0.20, most basic, temperature:
25 °C | [color ]
clear clear, colorless | [Appearance]
beige solid | [λmax]
365 nm (MeOH) | [Biological Applications]
Detecting bacteria/microorganisms; nitric oxide indicator, |
| Hazard Information | Back Directory | [Description]
DAF-FM diacetate (DAF-FM DA) is a cell-permeant, fluorescent probe for detecting and quantifying low concentrations of nitric oxide (NO). DAF-FM DA passively diffuses across cellular membranes and, once inside cells, is deacetylated by intracellular esterases and converted to cell-impermeant form — DAF-FM.
The fluorescence quantum yield of DAF-FM is ~0.005, but it increases about 160-fold to ~0.81 after reacting with NO and forming a fluorescent benzotriazole (excitation/emission maxima at 495/515 nm) .
The NO detection limit of DAF-FM (~3 nM) is more sensitive than that of DAF-2 (~5 nM). The fluorescence of the NO adduct of DAF-FM is independent of pH above pH 5.5. Moreover, the NO adduct of DAF-FM demonstrates a significantly enhanced photostability compared to that of DAF-2, ensuring reliable results and additional time for imaging.
DAF-FM DA should be dissolved in DMSO and then used to prepare a working solution. Buffers containing bovine serum albumin (BSA) or phenol red can affect the fluorescence and should be used cautiously. | [Uses]
DAF-FM diacetate is an important reagent for quantification of low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole. With excitation/emission maxima of 495 | [Biochem/physiol Actions]
DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases. | [in vivo]
Using sections of zebrafish embryos live-stained with DAF-FM DA (5 μM), could confirm that the fluorescent signals were predominantly located in areas of ongoing bone formation[1]. | [storage]
Store at -20°C | [Excitation / Emission Maximum]
495 nm/515 nm |
|
| Company Name: |
Sigma-Aldrich
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021-61415566 800-8193336 |
| Website: |
https://www.sigmaaldrich.cn |
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