| Identification | More | [Name]
4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE | [CAS]
333338-18-4 | [Synonyms]
4-NITROPHENYL DISODIUM ORTHOPHOSPHATE HEXAHYDRATE
4-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE
4-NITROPHENYLPHOSPHORIC ACID DISODIUM SALT HEXAHYDRATE
4-NPP, DISODIUM SALT, HEXAHYDRATE
DI-SODIUM 4-NITROPHENYL PHOSPHATE
DISODIUM 4-NITROPHENYL PHOSPHATE HEXAHYDRATE
DISODIUM P-NITROPHENYLPHOSPHATE HEXAHYDRATE
PHOSPHATASE SUBSTRATE
PHOSPHATASE SUBSTRATE DISODIUM SALT HEXAHYDRATE
P-NITROPHENYL PHOSPHATE DISODIUM HEXAHYDRATE
P-NITROPHENYL PHOSPHATE DISODIUM SALT 6H2O
P-NITROPHENYL PHOSPHATE DISODIUM SALT HEXAHYDRATE
PNPP
PNPP DISODIUM HEXAHYDRATE
PNPP DISODIUM SALT HEXAHYDRATE
PNP-PHOS DINA HEXAHYDRATE
PNP PHOSPHATE DISODIUM SALT HEXAHYDRATE
PNPP-NA | [EINECS(EC#)]
224-246-5 | [Molecular Formula]
C6H16NNa2O12P | [MDL Number]
MFCD00066288 | [Molecular Weight]
371.14 | [MOL File]
333338-18-4.mol |
| Chemical Properties | Back Directory | [Melting point ]
>300 °C(lit.) | [storage temp. ]
2-8°C
| [solubility ]
H2O: 25 mg/mL at 20 °C, clear, yellow-green
| [form ]
tablets
| [color ]
white to pale yellow
| [PH]
pH (50g/l, 25℃) : 8.0～9.0 | [biological source]
synthetic (organic) | [Water Solubility ]
almost transparency | [BRN ]
5324661 | [InChI]
1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2 | [InChIKey]
KAKKHKRHCKCAGH-UHFFFAOYSA-L | [SMILES]
O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1 | [CAS DataBase Reference]
333338-18-4(CAS DataBase Reference) |
| Safety Data | Back Directory | [Safety Statements ]
S24/25:Avoid contact with skin and eyes . | [WGK Germany ]
3
| [F ]
8-10-21 | [HS Code ]
29199000 | [Storage Class]
11 - Combustible Solids |
| Hazard Information | Back Directory | [Chemical Properties]
White to off-white powder | [Uses]
4-Nitrophenyl phosphate disodium salt hexahydrate has been used in:
- enzyme-linked immunosorbent assay (ELISA) to visualize glycoprotein VI (GPVI) binding to collagen type I (Col I)
- enzymatic color reaction for semi-quantitative measurement of allergen-specific IgE by ELISA
- alkaline phosphatase assay
| [Uses]
Phosphatase substrate has been used in the chromogenic detection of adsorbed rabbit IgG to determine coating efficiency. | [Uses]
Substrate for alkaline and acid phosphatase determinations4-Nitrophenyl phosphate disodium salt hexahydrate is a chromogenic substrate used in the determination of acid and alkaline phosphatases. | [General Description]
Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm. | [Biochem/physiol Actions]
4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP yields p-nitrophenol. pNPP is a common chromogenic substrate for alkaline phosphatase. |
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