| Identification | More | [Name]
6-CARBOXYFLUORESCEIN DIACETATE | [CAS]
3348-03-6 | [Synonyms]
6-CARBOXY-DI-O-ACETYLFLUORESCEIN
6-CARBOXYFLUORESCEIN DIACETATE
6-CARBOXYFLUORESCEINE DIACETATE
6-CFDA
6-FAM DIACETATE
6-Carboxy-di-O-acetylfluorescein, 6-CFDA
3',6'-Bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-6-carboxylic acid
3',6'-Diacetoxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-6-carboxylic acid
3-Oxo-3',6'-diacetoxyspiro[isobenzofuran-1(3H),9'-[9H]xanthene]-6-carboxylic acid | [Molecular Formula]
C25H16O9 | [MDL Number]
MFCD00036871 | [Molecular Weight]
460.39 | [MOL File]
3348-03-6.mol |
| Chemical Properties | Back Directory | [Melting point ]
152–153°C | [Boiling point ]
701.6±60.0 °C(Predicted) | [density ]
1.57±0.1 g/cm3(Predicted) | [storage temp. ]
2-8°C
| [solubility ]
DMSO: soluble
| [form ]
Solid | [pka]
6.4(at 25℃) | [color ]
Light yellow or off-white solid | [PH Range]
Weak green ' uorescence (6.0) to strong green ' uourescence (7.2) | [λmax]
<300nm | [Biological Applications]
Analyzing/detecting
cells;counting/
detecting/evaluating microorganisms;detecting/
measuring bacteria;quantifying adhesion of
tumor cells to endothelial monolayers;
measuring
gas (carbon dioxide or ammonia) concentration
in body luids;
monitoring intracellular nitric
oxide production;screening xenobiotic phloem
mobility in plants;
, studying low-density
lipoprotein (LDL)/cholesterol metabolism;apoptosis
assay;method for antifungal susceptibility
testing;
, as a substrate for measuring esterase
activity
| [Major Application]
Fluorescent probes, detecting cells, microorganisms, yeasts, antifungal agent, antibacterials agent | [InChI]
1S/C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25/h3-11H,1-2H3,(H,28,29) | [InChIKey]
QMOGCCYGOPYYNT-UHFFFAOYSA-N | [SMILES]
CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccc(cc45)C(O)=O)c1 | [CAS DataBase Reference]
3348-03-6(CAS DataBase Reference) |
| Hazard Information | Back Directory | [Description]
6-Carboxyfluorescein diacetate is a membrane-permeant prodrug of 6-Carboxyfluorescein, which has been used to differentiate viable cells from apoptotic cells. | [Uses]
6-Carboxyfluorescein Diacetate is a fluorescent polyanionic probe used to detect changes intracellular pH. In addition, 6-Carboxyfluorescein Diacetate derivatives can be used as staining agents for flow cytometry to visualize cells undergoing apoptosis or necrosis. | [Uses]
6-CARBOXYFLUORESCEIN DIACETATE is used to differentiate viable cells from apoptotic cells. Live cells that are not apoptotic accumulate 6-carboxyfluorescein but do not accumulate annexin
| [Excitation / Emission Maximum]
553 nm/575 nm |
|
|