| Identification | Back Directory | [Name]
EC 3.2.1.8 | [CAS]
37278-89-0 | [Synonyms]
AU-PE89
Belfeed
Bruzyme
XYLANASE
Asperzyme
Cartazyme
EC 3.2.1.8
BioBrite EB
Buzyme 2511
Buzyme 2524
Cartazyme HS
Biobake Silk
Biobrite HTX
Biobrite UHB
Bleachzyme F
Avizyme 1310
Cartazyme HT
Pentopan Mono
MIXEDXYLANASE
Albazyme 40S4X
Biobake CX 160
Belfeed B1100ML
Belfeed B1100MP
Cartazyme HS 10
Cartazyme NS 10
Cartazyme 9704E
Xylanase Enzyme
Cartazyme NST 10
Pentopan Mono BG
1,4-beta-Xylanase
Biobake Optum 500
endo-1,4-β-Xylanase
Pentopan Mono BG(R)
endo-1,4-β-D-Xylanase
XYLANASE, RECOMBINANT
EC 3.2.1.8 USP/EP/BP
Xylanase Trichoderma viride
1,4-β-D-Xylanxylanohydrolase
1,4-beta-Xylan endohydrolase
fromtrichodermaviride~2.5u/mg
1,4-BETA-D-XYLANXYLANOHYDROLASE
xylanase from thermomyces lanuginosus
xylanase from aureobasidium pullulans
Xylanase ex. Aspergillus Niger, 60MU/g
XYLANASE, RECOMBINANT FROM*THERYOMYCES L
xylanase from trichoderma viride ~2.5 U/mg
endo-1,4-β-Xylanase from Trichoderma longibrachiatum
Xylanase for Mash Viscosity Reduction and Xylan hydrolysis
XYLANASEFROMTHERMOMYCESLANUGINOSUSEXPRESSEDINFUSARIUMVENENATUM
Xylanase powder, >=2500 units/g, recombinant, expressed in Aspergillus oryzae | [EINECS(EC#)]
253-439-7 | [MDL Number]
MFCD00132594 |
| Hazard Information | Back Directory | [Uses]
Xylanase?has been used:
- in the isolation of hemicellulose and production of oligosaccharides
- in viscosimetric assay
- in the comparison of commercial enzymes for the aqueous enzymatic extraction of corn oil from corn germ
- in the hydrolytic degradation of xylan
| [General Description]
This product has been enhanced for waste prevention when used in cellulosic ethanol research. | [Biochem/physiol Actions]
Primary activity is an acid-neutral endo-1,4-β-D-xylanase, additional activities include β-glucanase, cellulase, pectinase, mannanase, xyloglucanase, laminarase, β-glucosidase, β-xylosidase, α-L-arabinofuranosidase, amylase, and protease. | [Purification Methods]
This xylanase is purified by anion-exchange chromatography on an Accell QMA column and finally by HPLC using a ProteinPak DEAE 5PW anion-exchange column. Solutions are stored frozen at -70o. [Morosoli et al. Biochem J 239 587 1986, Wong et al. Microbiol Rev 52 305 1988.] Carotenoids are polyene pigments that are mostly naturally occurring in bacteria, plants and animals. They have been isolated from the natural sources and obtained first by extraction with solvents and then purified by column chromatography through Al2O3 of various grades, Ca(OH)2 alone or with CaCO3,,,MgO or Silica Gel and eluted with solvents of various polarities. The progress of separation can be followed visually because the bands of most carotenoids are of various colours. The bands can be collected by elution, or the column can be extruded and the bands cut out and extracted with a polar solvent, e.g. MeOH. This chromatography can be repeated with the separate bands, and finally the carotenoids are recrystallised to analytical purity. The purity can be checked by TLC on Silica Gel or Al2O3 plates or paper chromatography and eluted in two dimensions. Gas-liquid or HPLC has been used for preparative work as well as for checking the purity and identifying them using internal standards such as tocopherol acetate (vitamin E acetate) and retinyl acetate. Carotenoids are generally light sensitive, easily oxidised by air and are affected by traces of acid, e.g. in solvents. These cause the polyenes to bleach or polymerize. The necessary precautions are therefore required to minimize these effects during isolation, purification and storage. They are identified by their UV-VIS spectra, and their molar extinction coefficients at specific wavelengths ( max) have been used for characterisation and for quantitation. More recently ORD, CD, NMR, IR and mass spectroscopy have been used extensively. Bibliography Carotenoids: Karrer & Jucker, Elsevier, 1950. The Comparative Biochemistry of the Carotenoids: Goodwin, Chapman & Hall Ltd, 1952. |
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