| Identification | More | [Name]
HYDROETHIDINE | [CAS]
38483-26-0 | [Synonyms]
DHE
HYDROETHIDINE
DIHYDROETHIDIUM
reducedethidium
HYDROETHIDINE(TM)
Dihydroethidium (DHE)
Hydroethidine [Dihydroethidium] *CAS 38483-26-0*
5,6-dihydro-3,8-diamino-5-ethyl-6-phenyl-phenanthridin
3,8-DIAMINO-5,6-DIHYDRO-5-ETHYL-6-PHENYLPHENANTHRIDINE
5,6-dihydro-3,8-diamino-5-ethyl-6-phenylphenanthridine
HYDROETHIDINE [DIHYDROETHIDIUM] *5 MM SOLUTION IN DMSO*
2,7-DIAMINO-10-ETHYL-9-PHENYL-9,10-DIHYDROPHENANTHRIDINE
Anti-Activin Receptor IIB antibody produced in goat | [EINECS(EC#)]
604-604-1 | [Molecular Formula]
C21H21N3 | [MDL Number]
MFCD00077335 | [Molecular Weight]
315.41 | [MOL File]
38483-26-0.mol |
| Safety Data | Back Directory | [Hazard Codes ]
Xn | [Risk Statements ]
R36/37/38:Irritating to eyes, respiratory system and skin .
R22:Harmful if swallowed. | [Safety Statements ]
S37/39:Wear suitable gloves and eye/face protection .
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice . | [WGK Germany ]
3
| [F ]
8-10 |
| Hazard Information | Back Directory | [Description]
Dihydroethidium is a cell-permeable blue fluorescent dye, which intercalates with nucleic acids and emits a red fluorescence detectable qualitatively by fluorescent microscopy or quantitatively by HPLC. It is a superoxide indicator. It exhibits blue fluorescence in the cytosol until oxidizedto ethidium, where it intercalates within the cell's DNA, staining its nucleus a bright fluorescent red. It is neuroprotective by reducing superoxide in mice after stroke. It has been used to detect reactive oxygen species during the phagocytic respiratory burst and for the detection of intracellular superoxide in cultured cells. | [Uses]
Dihydroethidium, also known as hydroethidine, is a dye that can permeate viable cells and accumulate in the nucleus after it is dehydrogenated to ethidium bromide and is used to measure oxygen reduction in phagocytes during respiratory burst. In the presence of an oxidant it will fluoresce and has been used as a dye in flow cytometry. Studies have used Dihydroethidium to study alveolar macrophages, blood leukocytes, and epithelial cells, among others, and has be used to measure intracellular H2O2 and O2-, and reactive oxygen. Dyes and metabolites. | [General Description]
A cell-permeable, chemically reduced ethidium derivative that can be used as a fluorogenic probe for the detection of reactive oxygen species (ROS). It undergoes significant oxidation in resting leukocytes, possibly through the uncoupling of mitochondrial oxidative phosphorylation. Cytosolic dihydroethidium displays blue fluorescence, however, following oxidation to ethidium and subsequent intercalation to DNA, a bright red fluorescence is achieved. Often used to determine the level of superoxide anion in cells by flow cytometry. | [Biochem/physiol Actions]
Cell permeable: yes | [Excitation / Emission Maximum]
490 nm/590 nm |
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