ChemicalBook--->CAS DataBase List--->615-28-1

615-28-1

615-28-1 Structure

615-28-1 Structure
IdentificationBack Directory
[Name]

OPD EASY-tablets
[CAS]

615-28-1
[Synonyms]

OPD
OPD-HC1
OPD 2HCL
O-PD, HCL
usafek-678
OPD TABLET
SIGMAFAST? OPD
OPD EASY-tablets
SIGMAFASTTM OPD
OPD DIHYDROCHLORIDE
OPD PEROXIDASE SUBSTRATE
1,2-Phenylenediamine2HCl
2-AMINOANILINE DIHYDROCHLORIDE
O-phenyldiaMine dihydrochloride
O-PHENYLENEDIAMMONIUM DICHLORIDE
o-phenylenediamine hydrochloride
o-Phenylenediamine·2hydrochloride
1,2-BENZENEDIAMINE DIHYDROCHLORIDE
O-PHENYLENEDIAMINE DIHYDROCHLORIDE
1,2-Phenylenediammonium dichloride
1,2-DIAMINOBENZENE DIHYDROCHLORIDE
1,2 PHENYLENDIAMINE DIHYDROCHLORIDE
benzene-1,2-diaMine dihydrochloride
1,2-PHENYLENEDIAMINE DIHYDROCHLORIDE
ORTHO-PHENYLENEDIAMINEDIHYDROCHLORIDE
1,2-BenzenediaMine,hydrochloride (1:2)
OPD EASY-tablets, for ELISA, 2mg/tablet
1,2-PhenylenediamineDihydrochloride99+%
o-Phenylenediamine dihydrochloride, 99+%
1,2-Phenylenediamine dihydrochloride p.a.
o-PHENYLENEDIAMINE DIHYDROCHLORIDE TABLETS
o-Phenylenediamine dihydrochloride(OPD HCl)
The adjacent benzene twoaMine hydrochloride
o-PhenylenediaMine dihydrochloride, 99+% 25GR
O-PHENYLENEDIAMINE TABLETS 20 MG*DIHYDRO CHLORIDE
O-PHENYLENEDIAMINE DIHYDROCHLORIDE*TABLE TS, 60 MG
O-PHENYLENEDIAMINE DIHYDROCHLORIDE*TABLE TS 4 MG SUB
O-PHENYLENEDIAMINE DIHYDROCHLORIDE*TABLE T 1 MG, TRU
O-PHENYLENEDIAMINE DIHYDROCHLORIDE*TABLE TS 2MG SUBS
O-PHENYLENEDIAMINE TABLETS CONTAINS*10 M G SUBSTRATE
O-PHENYLENEDIAMINE DIHYDROCHLORIDE*TABLE TS 15 MG SU
O-PHENYLENEDIAMINE TABLETS 3 MG PER*TABL ET, TRU-MEA
O-PHENYLENEDIAMINE DIHYDROCHLORIDE*TABLE TS 30 MG OP
1,2-PHENYLENEDIAMINE DIHYDROCHL., PGE W. 50 TABL., 5 MG/TAB
1,2-PHENYLENEDIAMINE DIHYDROCHL., PGE W. 50 TABL., 2 MG/TAB
1,2-PHENYLENEDIAMINE DIHYDROCHL., PGE W. 50 TABL., 30 MG/TB
1,2-PHENYLENEDIAMINE DIHYDROCHL., PGE W. 50 TABL., 10 MG/TB
1,2-PhenylenediaMine Dihydrochloride [for BiocheMical Research]
O-PHENYLENEDIAMINE DIHYDROCHLORIDE TABLETS 5 MG SUBSTRATE PER TABLET, TRU-MEASURE CHEMICL
[EINECS(EC#)]

210-418-7
[Molecular Formula]

C6H10Cl2N2
[MDL Number]

MFCD00012966
[MOL File]

615-28-1.mol
[Molecular Weight]

181.06
Chemical PropertiesBack Directory
[Appearance]

white to light pink powder
[Melting point ]

258 °C (dec.)(lit.)
[storage temp. ]

2-8°C
[solubility ]

H2O: 1 tablet/10 mL, clear, colorless
[form ]

tablet
[color ]

white to off-white
[PH]

1.5
[Stability:]

Stable. Incompatible with strong oxidizing agents.
[Water Solubility ]

Slightly soluble in water; freely soluble in alcohol, chloroform or ether
[BRN ]

3912045
[InChI]

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
[InChIKey]

RIIWUGSYXOBDMC-UHFFFAOYSA-N
[SMILES]

Cl[H].Cl[H].Nc1ccccc1N
[IARC]

2B (Vol. 123) 2020
[EPA Substance Registry System]

1,2-Phenylenediamine dihydrochloride (615-28-1)
Hazard InformationBack Directory
[Chemical Properties]

white to light pink powder
[General Description]

Brownish-yellow crystals. Slightly soluble in water. Used in the manufacture of dye.
[Air & Water Reactions]

Slightly soluble in water.
[Reactivity Profile]

Acidic organic/inorganic salts, such as OPD EASY-tablets, are generally soluble in water. The resulting solutions contain moderate to high concentrations of hydrogen ions and have pH's of less than 7.0. They react as acids to neutralize bases. These neutralizations generate heat, but less or far less than is generated by neutralization of inorganic acids, inorganic oxoacids, and carboxylic acid. They usually do not react as either oxidizing agents or reducing agents but such behavior is not impossible. Many of these compounds catalyze organic reactions.
[Uses]

o-Phenylenediamine dihydrochloride has been used as a substrate for horseradish-peroxidase-conjugated secondary antibody in enzyme-linked immunosorbent assay (ELISA).
[Uses]

o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
[Uses]

o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
[Purification Methods]

Crystallise the salt from dilute HCl (60mL conc HCl, 40mL water, with 2g stannous chloride), after treatment of the hot solution with charcoal by adding an equal volume of conc HCl and cooling in an ice-salt mixture. The crystals are washed with a small amount of conc HCl and dried in a vacuum desiccator over NaOH. [Beilstein 13 IV 38.]
Safety DataBack Directory
[Hazard Codes ]

Xn,N,T
[Risk Statements ]

22-40-43-51/53-68-50/53-36-25-20/21-36/37/38
[Safety Statements ]

36/37-61-60-45-28-26
[RIDADR ]

UN 2811 6.1/PG 3
[WGK Germany ]

3
[RTECS ]

ST0175000
[F ]

8-10-23
[TSCA ]

Yes
[HazardClass ]

6.1(b)
[PackingGroup ]

III
[HS Code ]

29215190
[Storage Class]

11 - Combustible Solids
[Hazard Classifications]

Aquatic Chronic 2
Carc. 2
Muta. 2
Skin Sens. 1
[Hazardous Substances Data]

615-28-1(Hazardous Substances Data)
Spectrum DetailBack Directory
[Spectrum Detail]

OPD EASY-tablets(615-28-1)1HNMR
OPD EASY-tablets(615-28-1)13CNMR
OPD EASY-tablets(615-28-1)IR1
OPD EASY-tablets(615-28-1)IR2
OPD EASY-tablets(615-28-1)IR3
OPD EASY-tablets(615-28-1)Raman
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