| Identification | Back Directory | [Name]
Dianemycin 1-sodium salt | [CAS]
65101-87-3 | [Synonyms]
CS-2194
NanchangMycin
NANCHANGMYCIN A
Dianemycin 1-sodium salt
Nanchangmycin (Nanchangmycin A)
Dianemycin 1-sodium salt USP/EP/BP
(2S,4R,6E,8S)-8-[(2S,2'R,4'S,5R,5'S,7R,7'R,8R,9S,9'S,10'R)-9-Hydroxy-2,4',8,10'-tetramethyl-2'-[(2S,3S,5R,6R)-tetrahydro-6-hydroxy-6-(hydroxymethyl)-3,5-dimethyl-2H-pyran-2-yl]-9'-[[(2S,5S,6R)-tetrahydro-5-methoxy-6-methyl-2H-pyran-2-yl]oxy][2,7'-bi-1,6-dioxaspiro[4.5]dec]-7-yl]-2,4,6-trimethyl-5-oxo-6-nonenoic acid sodium salt
6-Nonenoic acid, 8-[(2S,2'R,4'S,5R,5'S,7R,7'R,8R,9S,9'S,10'R)-9-hydroxy-2,4',8,10'-tetramethyl-2'-[(2S,3S,5R,6R)-tetrahydro-6-hydroxy-6-(hydroxymethyl)-3,5-dimethyl-2H-pyran-2-yl]-9'-[[(2S,5S,6R)-tetrahydro-5-methoxy-6-methyl-2H-pyran-2-yl]oxy][2,7'-bi-1,6-dioxaspiro[4.5]dec]-7-yl]-2,4,6-trimethyl-5-oxo-, sodium salt (1:1), (2S,4R,6E,8S)- | [Molecular Formula]
C47H77NaO14 | [MDL Number]
MFCD02874368 | [MOL File]
65101-87-3.mol | [Molecular Weight]
889.096 |
| Questions And Answer | Back Directory | [Preparation]
method for producing Dianemycin 1-sodium salt using *Streptomyces nanchangensis* fermentation includes spore culture, seed tank culture, fermenter culture, and extraction steps:
1) *Streptomyces nanchangensis* preserved in sand tubes are inoculated onto Gao's medium or bran agar medium for scale-up culture for 10-20 days;
2) *Streptomyces nanchangensis* spores obtained in step 1) are inoculated into a seed tank containing the seed medium designed in this invention and equipped with a stirring device for fermentation culture. The fermentation culture conditions are: temperature: 25-35℃; aeration rate: 1:0.5-1:1.5V/V; stirring speed: 200-600 rpm; culture time: 36-60 hours.
3) The culture obtained in step 2) is further inoculated into a fermenter containing the culture medium designed in this invention and equipped with a stirring device for fermentation culture. The fermentation culture conditions are: inoculum size 5-20% V/V; temperature 25-35℃; aeration rate 1:0.5-1.5 V/V; stirring speed 200-600 rpm; fermentation time 90-140 hours. Multiple intermediate feedings can be made during fermentation in the fermenter, and the fermentation time is 120-230 hours in this case;
4) The fermentation liquid obtained in step 3) is filtered to obtain filtrate and filter cake;
5) The filter cake is extracted with methanol, ethanol, chloroform, dichloromethane, acetone or benzene to obtain an extract;
6) The extract is concentrated, and then the concentrated extract is extracted with ethyl acetate or butyl acetate to obtain an oily substance;
7) The oily substance is absorbed with excipients, dried, ground into powder, and the Dianemycin 1-sodium salt preparation is obtained.
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| Chemical Properties | Back Directory | [storage temp. ]
Store at -20°C | [solubility ]
DMF: 30 mg/mL; DMSO: 25 mg/mL; Ethanol: 25 mg/mL | [form ]
A crystalline solid | [color ]
White to off-white | [InChIKey]
XMAIRYYXDCNFKP-NXQQWTHSNA-M |
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| Company Name: |
cjbscvictory
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| Telephone: |
13348960310 13348960310 |
| Website: |
www.weikeqi-biotech.com/ |
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