| Identification | More | [Name]
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt | [CAS]
6578-06-9 | [Synonyms]
5-BROMO-4-CHLORO-1H-INDOL-3-YL PHOSPHATE P-TOLUIDINE
5-BROMO-4-CHLORO-3-INDOLYL-PHOSPHATE 4-TOLUIDINE SALT
5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE, COMPOUNDED WITH 4-METHYLBENZENAMINE
5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE P-TOLUIDINE
5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE P-TOLUIDINE SALT
5-BROMO-4-CHLORO-3'-INDOLYPHOSPHATE P-TOLUIDINE SALT
5-BROMO-4-CHLORO-3-INDOXYL PHOSPHATE, P-TOLUIDINE SALT
5-BROMO-4-CHLORO-3-INDOYL PHOSPHATE, P-TOLUIDINE SALT
BCIP
BCIP 4-TOLUIDINE SALT
BCIP/INT SUBSTRATE
BCIP PARA-TOLUIDINE SALT
BCIP P-TOL
BCIP P-TOLUIDINE
BCIP P-TOLUIDINE SALT
BCIP-T
BCIP-TOLUIDINE
BROMO-4-CHLORO-3-INDOLYL PHOSPHATE MONO-(-TOLUIDINIUM) SALT
PHOSPHORIC ACID-MONO-(5-BROMO-4-CHLORO-1H-INDOL-3-YL) ESTER P-TOLUIDINE
X-PHOSPHATE P-TOLUIDINE SALT | [EINECS(EC#)]
229-506-1 | [Molecular Formula]
C22H24BrClN3O4P | [MDL Number]
MFCD00040636 | [Molecular Weight]
540.77 | [MOL File]
6578-06-9.mol |
| Chemical Properties | Back Directory | [Melting point ]
194-195℃ | [storage temp. ]
2-8°C
| [solubility ]
DMF: 20 mg/mL
| [form ]
tablets
| [color ]
White to Orange to Green | [InChI]
InChI=1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3 | [InChIKey]
QEIFSLUFHRCVQL-UHFFFAOYSA-N | [SMILES]
C12C(Cl)=C(C=CC=1NC=C2OP(=O)(O)O)Br.C1(C)C=CC(N)=CC=1 | [CAS DataBase Reference]
6578-06-9(CAS DataBase Reference) | [EPA Substance Registry System]
6578-06-9(EPA Substance) |
| Safety Data | Back Directory | [Symbol(GHS) ]
  
GHS02,GHS07,GHS08 | [Signal word ]
Danger | [Hazard statements ]
H226-H312+H332-H319-H360D | [Precautionary statements ]
P201-P210-P261-P280-P308+P313-P370+P378 | [Hazard Codes ]
Xi | [Risk Statements ]
R36/37/38:Irritating to eyes, respiratory system and skin .
R40:Limited evidence of a carcinogenic effect. | [Safety Statements ]
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice . | [RIDADR ]
2811 | [WGK Germany ]
3
| [F ]
3-8-10 | [TSCA ]
TSCA listed | [HazardClass ]
6.1 | [PackingGroup ]
III | [HS Code ]
29349990 | [Storage Class]
11 - Combustible Solids |
| Hazard Information | Back Directory | [Chemical Properties]
White to light brown powder | [Uses]
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid. | [General Description]
BCIP serves as substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually detected. | [Biochem/physiol Actions]
5-bromo-4-chloro-3-indolyl phosphate (BCIP) can be used to visualize the phosphatase activity. It can be used as a substrate to monitor secreted embryonic alkaline phosphatase?activity in solution. |
|
| Company Name: |
J & K SCIENTIFIC LTD.
|
| Telephone: |
18210857532 18210857532 |
| Website: |
https://www.jkchemical.com |
| Company Name: |
Energy Chemical
|
| Telephone: |
400-0056266 |
| Website: |
www.energy-chemical.com |
|