| Identification | Back Directory | [Name]
MCA-LYS-PRO-LEU-GLY-LEU-DAP(DNP)-ALA-ARG-NH2 | [CAS]
720710-69-0 | [Synonyms]
Mca-KPLGL-Dap(Dnp)-AR-NH2
MMPs substrate Fluorogenic
MCA-LYS-PRO-LEU-GLY-LEU-DAP(DNP)-ALA-ARG-NH2
MOCAC-LYS-PRO-LEU-GLY-LEU-DAP(DNP)-ALA-ARG-NH2
Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2Trifluoroacetate salt
N2-[2-(7-Methoxy-2-oxo-2H-1-benzopyran-4-yl)acetyl]-L-lysyl-L-prolyl-L-leucylglycyl-L-leucyl-3-[(2,4-dinitrophenyl)amino]-L-alanyl-L-alanyl-L-argininamide
L-Argininamide, N2-[2-(7-methoxy-2-oxo-2H-1-benzopyran-4-yl)acetyl]-L-lysyl-L-prolyl-L-leucylglycyl-L-leucyl-3-[(2,4-dinitrophenyl)amino]-L-alanyl-L-alanyl- | [Molecular Formula]
C55H80N16O16 | [MDL Number]
MFCD08458655 | [MOL File]
720710-69-0.mol | [Molecular Weight]
1221.32 |
| Chemical Properties | Back Directory | [form ]
Lyophilized yellow solid. | [color ]
Light yellow to yellow | [Appearance]
Lyophilized Yellow Solid | [Sequence]
{Mca}-Lys-Pro-Leu-Gly-Leu-{Dap(Dnp)}-Ala-Arg-NH2 |
| Hazard Information | Back Directory | [Description]
Mca-KPLGL-Dpa-AR-NH2 is an N-terminal elongation of the widely used MMP FRET substrate Mca-PLGL-Dpa-AR-NH2 with a Lys yields a fluorogenic peptide with improved substrate properties. Compared with Mca-PLGL-Dpa-AR-NH2, the specificity constant (kcat/Km) of the broad-spectrum MMP-substrate Mca-KPLGL-Dpa-AR-NH2 for collagenases (MMP-1, MMP-8, MMP-13) and MMP-14 is increased by 2-9 times and 3 times, respectively, while those for gelatinases and matrilysin remain equally high. | [Uses]
Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 (FS-6) is a fluorescent peptide that is a quenched MMP peptide substrate. Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 can be used for real-time quantification of MMP enzymatic activity. Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 is an elongated peptide of MMP substrate (FS-1) and is active against collagenases (MMP-1, MMP-8, MMP-13 ) and MT1-MMP with higher specificity constants than FS-1[1]. (Ex/Em=325 nm/400 nm) | [IC 50]
MMP-1: 27.5 μM (Km); MMP-8; MMP-13: 5.2 μM (Km); MMP-14: 7.9 μM (Km) | [References]
[1] Neumann U, et al. Characterization of Mca-Lys-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2, a fluorogenic substrate with increased specificity constants for collagenases and tumor necrosis factor converting enzyme. Anal Biochem. 2004 May 15;328(2):166-73. DOI:10.1016/j.ab.2003.12.035 | [Sequene]
Mca-KPLGL-Dpa-AR-NH2 |
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