| Identification | Back Directory | [Name]
2'-(4-METHYLUMBELLIFERYL)-ALPHA-D-N-ACETYLNEURAMINIC ACID SODIUM SALT | [CAS]
76204-02-9 | [Synonyms]
NEU5AC-A-4MU
4-MU-NANA NA
Neu5Ac-α-4MU
NEU5AC-ALPHA-4MU SODIUM SALT
2-(4-methylumbelliferyl)-A-D-N-*acetylneuraminic
2-(4-methylumbelliferyl)-α-D-N-*acetylneuraminic
2'-(4-METHYLUMBELLIFERYL)-A-D-N-*ACETYLN EURAMINIC A
4-methylumberiferryl-N-acetyl-alpha-D-neuraminic acid Na
4-MethylumbelliferylN-acetyl-a-D-neuraminicacidsodiumsalt
4-methylumbelliferyl-N-acetyl-alpha-D-neuram.A.so-S.dihy.
4-Methylumbelliferyl N-acetyl-α-D-neuraminic acid sodium salt
4-Methylumbelliferyl-N-acetyl-alpha-D-neuraminic acid sodium salt
2'-(4-METHYLUMBELLIFERYL)-A-D-N-ACETYLNEURAMINIC ACID, SODIUM SALT
2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic Acid, Sodium Salt
4-Methylumbelliferyl N-acetyl-a-D-neuraminic acid sodium salt ,97%
2'-(4-METHYLUMBELLIFERYL)-ALPHA-D-N-ACETYLNEURAMINIC ACID SODIUM SALT
4-methylumbelliferyl-n-acetyl-α-d-neuraminic acid sodium salt hydrate
4-Methylumbelliferyl-N-acetyl-a-D-neuraminic acid Sodium salt Dihydrate
2'-(4-METHYLUMBELLIFERYL)-A-D-N-ACETYLNEURAMINIC ACID SODIUM SALT HYDRATE
2'-(4-methylumbelliferyl)-α-d-n-acetylneuraminic acid sodium salt hydrate
4-Methylumbelliferyl-N-acetyl-α-D-neuraminic acid hydrate sodium salt
2μ-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid hydrate sodium salt
2'-(4-Methylumbelliferyl)-alpha-D-N-acetylneuraminic acid sodium salt hydrate
N-Acetyl-2-O-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-alpha-neuraminic acid monosodium salt | [EINECS(EC#)]
1533716-785-6 | [Molecular Formula]
C21H24NNaO11 | [MDL Number]
MFCD00057309 | [MOL File]
76204-02-9.mol | [Molecular Weight]
489.41 |
| Chemical Properties | Back Directory | [Appearance]
Off-White to Yellow Solid | [Melting point ]
171°C dec. | [storage temp. ]
−20°C
| [solubility ]
H2O: 50 mg/mL, clear, very slightly yellow
| [form ]
Solid | [color ]
White to Off-White | [Water Solubility ]
water: 50mg/mL, clear to slightly hazy, colorless to light yellow | [InChIKey]
NSQMRVBWXQQIKF-NVRWCLOTSA-M | [SMILES]
O.[H]C(O)(CO)[C@]([H])(O)[C@@H]1OC(C[C@H](O)[C@H]1NC(C)=O)(Oc2ccc3C(C)=CC(=O)Oc3c2)C(=O)O[Na] |
| Hazard Information | Back Directory | [Chemical Properties]
Off-White to Yellow Solid | [Uses]
This compound has been used for fluorometric assay of neuraminidase as well as fluorescent staining of sialidases in PAGE. | [Uses]
Used as a substrate in a fluorometric assay of neuraminidase. | [Description]
4-Methylumbelliferyl-N-acetyl-α-D-neuraminic acid is a fluorogenic substrate of neuraminidases.1 4-Methylumbelliferyl-N-acetyl-α-D-neuraminic acid is cleaved by neuraminidases to release the fluorescence moiety 4-methylumbelliferyl (4-MU). 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH, respectively, and an emission maximum ranging from 445 to 455 nm, increasing as pH decreases. 4-Methylumbelliferyl-N-acetyl-α-D-neuraminic acid has been used to quantify neuraminidase activity in mammalian cells and clinical isolates of influenza virus.2,3,4 | [Biochem/physiol Actions]
Substrate for fluorometric assay of neuraminidase. Used for fluorescent staining of sialidases in PAGE. | [References]
[1] E MONTI. Expression of a novel human sialidase encoded by the NEU2 gene.[J]. Glycobiology, 1999, 9 12: 1313-1321. DOI: 10.1093/glycob/9.12.1313
[2] NICHOLAS M STAMATOS. Differential expression of endogenous sialidases of human monocytes during cellular differentiation into macrophages.[J]. FEBS Journal, 2005, 272 10: 2545-2556. DOI: 10.1111/j.1742-4658.2005.04679.x
[3] ALAN S CROSS. NEU1 and NEU3 sialidase activity expressed in human lung microvascular endothelia: NEU1 restrains endothelial cell migration, whereas NEU3 does not.[J]. The Journal of Biological Chemistry, 2012: 15966-15980. DOI: 10.1074/jbc.m112.346817
[4] N T WETHERALL. Evaluation of neuraminidase enzyme assays using different substrates to measure susceptibility of influenza virus clinical isolates to neuraminidase inhibitors: report of the neuraminidase inhibitor susceptibility network.[J]. Journal of Clinical Microbiology, 2003, 41 2: 742-750. DOI: 10.1128/jcm.41.2.742-750.2003 |
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