| Identification | Back Directory | [Name]
JP83 | [CAS]
887264-44-0 | [Synonyms]
JP83
JMLPLSJWSHVJLP-UHFFFAOYSA-N
Carbamic acid, N-(6-phenylhexyl)-, 3'-(aminocarbonyl)[1,1'-biphenyl]-3-yl ester | [Molecular Formula]
C26H28N2O3 | [MDL Number]
MFCD12912315 | [MOL File]
887264-44-0.mol | [Molecular Weight]
416.51 |
| Chemical Properties | Back Directory | [storage temp. ]
Store at -20°C | [solubility ]
≤1mg/ml in ethanol;30mg/ml in DMSO;30mg/ml in dimethyl formamide | [form ]
crystalline solid | [color ]
White to off-white |
| Hazard Information | Back Directory | [Uses]
JP83 is an irreversible fatty acyl amide hydrolase (FAAH) inhibitor with an IC50 of 1.6 nM in competitive activity-based protein profiling (ABPP) experiments[1]. | [Definition]
ChEBI: JP83 is a member of biphenyls. | [Biological Activity]
jp83 is an irreversible fatty acyl amide hydrolase (faah) inhibitor.the enzyme fatty acyl amide hydrolase (faah) is capable of hydrolyzing anandamide and other esters and amides with long unsaturated acyl chains, which is widely expressed in brain and other tissues. | [in vitro]
jp83 was identified as an irreversible faah inhibitor of the carbamate class when it was tested using radiolabeled oleamide as the substrate. ms results indicated that it inhibited faah by carbamylation of the enzyme’s serine nucleophile. in addition, jp83 was found to be able to inhibit faah with equal or greater potency than urb597 [1]. | [in vivo]
mice were treated with jp104, a close analog of jp83, after which they were sacrificed and their tissues removed for click chemistry analysis. it was found that at 1 mg/kg of jp104, faah labeling was ~80% of maximum in the brain, while none of the liver and kidney targets were modified to greater than 20%. furthermore, the nearly complete inactivation of brain faah by jp104 at 1 mg/kg was confirmed by competitive abpp studies with fp-rh. in contrast, jp104 couldnot reduce the intensity of fp-rh signals in liver and kidney proteomes significantly [1]. | [IC 50]
14 nm for the human recombinant enzyme | [storage]
Store at -20°C | [References]
[1] alexander, j. p., and cravatt, b.f. mechanism of carbmate inactivation of faah: implications for the design of covalent inhibitors and in vivo functional probes for enzymes. chemistry & biology 12, 1179-1187 (2005). |
|
|