| Identification | Back Directory | [Name]
Collagenase | [CAS]
9001-12-1 | [Synonyms]
CLGN
Santyl
Azocollase
FIBROBLAST
Collagenase
COLLAGENESE
INTERSTITIAL
CollagenaseⅢ
Collagenase I
Collagenase V
Collagenase A
Soycollagestin
Collagenase II
Collagenase IV
CollagenaseIII
Collagen enzyme
Collagen-Peptide
Collagen protease
Collagen peptidase
COLLAGENASE TYPE X
COLLAGENASE TYPE S
Metallocollagenase
COLLAGENASE TYPE XI
COLLAGENASE TYPE V-S
collagenase type I-S
collagenase type vii
Aspergillopeptidase C
COLLAGENASE TYPE IA-S
COLLAGENASE TYPE IV-S
Clostridiopeptidase A
Clostridiopeptidase I
Clostridiopeptidase II
Matrix metalloprotease-1
COLLAGENASE NB 4G PROVED GRADE
Recombinant Collagenase G >95%
Recombinant Collagenase H >95%
COLLAGENASE SIGMA BLEND TYPE F
COLLAGENASE SIGMA BLEND TYPE H
COLLAGENASE SIGMA BLEND TYPE N
COLLAGENASE SIGMA BLEND TYPE L
COLLAGENASE TYPE III FRACTION A
COLLAGENASE N REFERENCE CONTENT
COLLAGENASE N REFERENCEIDENTITY
Recombinant Collagenase H/G >70%
Recombinant Collagenase H/G >95%
(VIAL) COLLAGENASE NB1 GMP GRADE
COLLAGENASE TYPE VII HIGH PURITY
Clostridium histolyticum collagenase
COLLAGENASE STERILE FILTERED TYPE XI-S
CollagenaseFormIiExClostridiumHistolyt-
CollagenaseFormIExClostridiumHistolyti-
collagenase F. clostridium histolyticum
collagenase from achromobacter iophagus
collagenase sterile filtered type vii-S
Collagenase from Clostridium histolyticum
COLLAGENASE TYPE IA-S CELL CULTURE TESTED
COLLAGENASE TYPE XI-S CELL CULTURE TESTED
COLLAGENASE TYPE VII-S CELLCULTURE TESTE D
COLLAGENASE NB1 FROM CL. HIST.PREMIUM GRADE
COLLAGENASE NB4 FROM CL. HIST.STANDARD GRADE
collagenase crude type ia cell culture*tested
COLLAGENASE CRUDE TYPE XI CELL CULTURETE STED
Collagenase, Type II, Clostridium histolyticum
COLLAGENASE NB6 FROM CL. HISTOLYTICUMGMP GRADE
COLLAGENASE NB 8 FROM CL. HISTOLYTICUMBROAD RANGE
Collagenase from Clostridium histolyticum(Type 1)
Collagenase from Clostridium histolyticum(Type 2)
Collagenase from Clostridium histolyticum(Type 4)
Collagenase from Clostridium histolyticum(Type 3)
Anti-MMP-1, C-Terminal antibody produced in rabbit
Collagenase from Clostridium histolyticum,lyophil.
COLLAGENASE FROM CLOSTRIDIUM HISTOLYTICUM, TYPE IV
COLLAGENASE FROM CLOSTRIDIUM HISTOLYTICUM, TYPE II
COLLAGENASE HIGH PURITY TYPE VII CELLCUL TURE TESTE
COLLAGENASE NB 5 FROM CL. HISTOLYTICUMSTERILE GRADE
Collagenase froM ClostridiuM histolyticuM(Purified)
Collagenase,Type-III,from Clostridium histolyticum
COLLAGENASE FROM CLOSTRIDIUM HISTOLYTICUM, TYPE VIII
Collagenase, Paralithodes camtschatica Hepatopancreas
COLLAGENASE FROM CLOSTRIDIUM HISTOLY-TIC UM, ~0.1 U/MG
Anti-MMP1 (Cleaved-Phe100) antibody produced in rabbit
COLLAGENASE FROM CLOSTRIDIUM HISTOLY-TIC UM, ~0.5-2 U/MG
COLLAGENASE F. CLOSTRIDIUM HISTOLYTICUM POWD.0.15-0.4 U/MG*
Collagenase from Clostridium histolyticum,Clostridiopeptidase A
Collagenase from Clostridium histolyticum(Animal Origin Free,A)
Collagenase from Clostridium histolyticum(Animal Origin Free,C)
Collagenase from Clostridium histolyticum(Animal Origin Free,B)
Collagenase NB 4G Proved Grade froM ClostridiuM histolyticuM crude
COLLAGENACOLLAGENASE FROM CLOSTRIDIUM HISTOLYTICUM, TYPE IA, FOR GENERAL USE
Collagenase from Clostridium histolyticum Type II, Vetec(TM) reagent grade, powder, >=125 CDU/mg solid
Collagenase from Clostridium histolyticum Type IV, Vetec(TM) reagent grade, powder, >=160 CDU/mg solid
Collagenase from Clostridium histolyticum Type I, for general use, Vetec(TM) reagent grade, powder, >=125 CDU/mg solid | [EINECS(EC#)]
232-582-9 | [Molecular Formula]
NULL | [MDL Number]
MFCD03456440 | [MOL File]
9001-12-1.mol | [Molecular Weight]
849.449 |
| Chemical Properties | Back Directory | [Definition]
A rare proteinase that catalyzes the hydrolysis of collagen. Collagenase activity has been demonstrated
in several venoms of crotalid and viperid snakes and
it occurs in certain species of clostridium. | [storage temp. ]
2-8°C
| [solubility ]
Soluble in aqueous buffers. | [form ]
powder
| [color ]
light brown
| [PH]
7.0 | [Merck ]
2481 | [CAS DataBase Reference]
9001-12-1 | [EPA Substance Registry System]
Collagenase(9001-12-1) |
| Hazard Information | Back Directory | [Uses]
This collagenase has been tested with cell lines to verify the product is not cytotoxic. Collagenase is typically used to digest the connective components in tissue samples to liberate individual cells. The concentration for cartilage dispersal is 1-2 mg/ml, but literature searches should be performed for species specific and/or tissue specific concentrations. | [General Description]
Collagenase from Clostridium histolyticum comprises of collagenases G and H. Collagenase has N-terminal activator domain and C-terminal peptidase domain. The collagen binding domains (CBD) and polycystic kidney disease-like (PKD-like) domain resides in the peptidase domain. The collagenase module undergoes structural changes upon calcium binding. | [Biochem/physiol Actions]
Effective release of cells from tissue requires the action of collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca2+) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA)4; β-mercaptoethanol; glutathione, reduced; thioglycolic acid, sodium; and 2,2′-dipyridyl; 8-hydroxyquinoline are known to inhibit the enzyme activity. | [Purification Methods]
Collagenase is purified by using N-ethylmaleimide to activate the enzyme, and wheat germ agglutinin-agarose affinity chromatography [Callaway et al. Biochemistry 25 4757 1986]. |
| Questions And Answer | Back Directory | [Description]
Collagenases are a category of enzymes that can digest native collagen, the major fibrous component of animal extracellular connective tissue. Collagenases can be produced by some bacterias such as Clostridium and can also be made by the animal body as part of its normal immune response. They normally target the connective tissue in muscle cells and other body organs. They can break peptide bonds within collagen and help create collagen by cleaving pro-collagen, collagen's precursor, once it is secreted by the cell. Bacterial collagenases exhibit broader substrate specificity than animal collagenases and unlike animal collagenases that split collagen in its native triple-helical conformation, bacterial collagenase can degrade both water-insoluble native collagens and water-soluble denatured ones. In addition, bacterial collagenase can attack almost all collagen types, and is able to make multiple cleavages within triple helical regions. Collagenases have a molecular weight ranging from 68 kilodaltons (kDa) to 130 Kda due to differing sizes based on the class to which the isolate belongs. The optimal pH for stability ranges from approximately 6.3 to 7.5 with a theoretical isoelectric point of 5.62. Numerous compounds and molecules have the capability of acting as collagenases activator or inhibitor. For example, Ca2+ and Zn2+ ions are two known activators. On the other hand, ethylenediaminetetraacetic acid (EDTA), ethylene glycol tetraacetic acid (EGTA), cysteine, histidine, dithiothreitol (DTT), 2-mercaptoethanol, o-phenanthroline, Hg2+, Pb2+, Cd2+, Cu2+, and Zn2+ are all known inhibitors of collagenase activity.
| [References]
1.https://en.wikipedia.org/wiki/Collagenase
2.http://www.wisegeek.com/what-is-collagenase.htm
3.http://www.rockland-inc.com/collagenase.aspx
4.http://www.worthington-biochem.com/cls/default.html
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