| Identification | Back Directory | [Name]
GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE | [CAS]
9001-50-7 | [Synonyms]
gapd
GAPDH
cb609
G-3-PDH
mg:bb02e05
EC 1.2.1.1.2
EC: 1.2.1.12
GAPDH)/CH8530
D-Glyceraldehyde 3-phosphate
Human GAPDH Protein, His Tag
Triosephosphate dehydrogenase
Glyceraldehyde-3-PhosphateDehydrog-
NAD+ oxidoreductase (phosphorylating)
Dehydrogenase, glyceraldehyde phosphate
phosphoglycerine aldehyde dehydrogenase
GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE
D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE
Glyceraldehyde-3-phosphate dehydrogenase human
Glyceraldehyde-3-phosphate dehydrogenase (NAD)
GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE USP/EP/BP
GLYCERALDEHYDE-3-PHOSPHATE*DEHYDROGENASE FROM RABBI
GLYCERALDEHYDE-3-PHOSPHATE*DEHYDROGENASE , FROM BAKE
GLYCERALDEHYDE-3-PHOSPHATE*DEHYDROGENASE FROM CHICK
Native Human Glyceraldehyde-3-phosphate Dehydrogenase
Native Rabbit Glyceraldehyde-3-phosphate Dehydrogenase
glyceraldehyde-3-phoshate dehydrogenase(rabbit muscle)
Native Chicken Glyceraldehyde-3-phosphate Dehydrogenase
glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)
glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle
glyceraldehyde-3-phosphate dehydrogenase from chicken muscle
GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM HUMAN ERYTHROCYTES
D-GLYCERALDEHYDE-3-PHOSPHATE: NAD OXIDOREDUCTASE (PHOSPHORYLATING)
D-GLYCERALDEHYDE 3-PHOSPHATE: NAD+ OXIDOREDUCTASE [PHOSPHORYLATING]
GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE, SUSPENSION, FROM RABBIT MUSCLE
glyceraldehyde-3-phosphate dehydrogenase from baker's yeast (s. cerevisiae)
Native Bakers yeast (S. cerevisiae) Glyceraldehyde-3-phosphate Dehydrogenase
GAPDH, D-Glyceraldehyde 3-phosphate:NAD+ oxidoreductase (phosphorylating)
Glyceraldehyde-3-phosphate Dehydrogenase Agarose from baker's yeast (S.cerevisiae)
Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle lyophilized powder, >=75 units/mg protein
Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle,D-Glyceraldehyde 3-phosphate:NAD+ oxidoreductase (phosphorylating), GAPDH
Glyceraldehyde-3-phosphate Dehydrogenase from chicken muscle,D-Glyceraldehyde 3-phosphate:NAD+ oxidoreductase (phosphorylating), GAPDH
Glyceraldehyde-3-phosphate Dehydrogenase from human erythrocytes,D-Glyceraldehyde 3-phosphate:NAD+ oxidoreductase (phosphorylating), GAPDH
Glyceraldehyde-3-phosphate Dehydrogenase from baker's yeast (S. cerevisiae),D-Glyceraldehyde 3-phosphate:NAD+ oxidoreductase (phosphorylating), GAPDH | [EINECS(EC#)]
232-609-4 | [Molecular Formula]
C3H7O6P | [MDL Number]
MFCD00131203 | [MOL File]
9001-50-7.mol | [Molecular Weight]
170.058 |
| Hazard Information | Back Directory | [Chemical Properties]
white crystalline substance suspended in a 3.2 mol/L ammonium sulfate solution (containing 0.1 mmol/L EDTA). Stability: The suspension can be stored at 5°C for several months without changing its activity. Regular replacement of the medium maintains activity over time. Freezing or thawing can inactivate it. Each molecule of glyceraldehyde-3-phosphate dehydrogenase contains 3.8-4.0 highly active cysteine residues and can bind to four molecules of coenzyme I (NAD+) or reduced coenzyme I (NADH). The enzymatic reaction: D-glyceraldehyde-3-phosphate + coenzyme I + inorganic phosphorus 1,3-diphospho-D-glycerate + reduced coenzyme I. | [Uses]
Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle has been used:
- for measurements of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity assay
- to generate a linear standard curve to analyse GAPDH activity in the experimental samples collected from mice
- in anti-aggregation assays
- in purified GAPDH studies
| [General Description]
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) catalyzes the conversion of glyceraldehyde-3-phosphate into D-glycerate-1,3-bisphosphate as part of the glycolysis pathway. GAPDH has also been found to function in additional cellular process, such as transcription, apoptosis, oxidative stress and ER to Golgi transport. | [Biochem/physiol Actions]
Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate as part of glycolysis. It has also been shown to have roles in initiation of apoptosis, transcription activation and the shuttling of ER to Golgi vesicles<<<,New>>>. | [Purification Methods]
Purify the dehydrogenase from rabbit muscle by extraction with 0.03N KOH and precipitate it with (NH4)2SO4 (0.52 of saturation). The clear supernatant is adjusted to pH 7.5, and NH3 is added dropwise to pH 8.2-8.4. Crystals appear sometimes even without seeding. The crystals are dissolved in H2O, filtered to remove suspended material and 2 volumes of saturated (NH4)2SO4 at pH 8.2-8.4 is added. After 1hour the crystals appear. Recrystallise it in the same way. [Cori et al. J Biol Chem 173 605 1948, Furfine & Velick J Biol Chem 240 844 1965, The Enzymes 7 243 1963, Lui & Huskey Biochemistry 31 6998 1992.] The Km values are: NADH (3.3WM) and 1,3-diphosphoglycerate (8x10-7M) in pH 7.4 imidazole buffer at 26o, NAD (13WM), glyceraldehyde-3-P (90WM), Pi (2.9x10-4M), and arsenate (69WM) in 8.6 M NaHCO3 buffer at 26oC. [Orsi & Cleland Biochemistry 11 102 1972.] |
|
| Company Name: |
Sigma-Aldrich
|
| Telephone: |
021-61415566 800-8193336 |
| Website: |
https://www.sigmaaldrich.cn |
|