| Identification | Back Directory | [Name]
Acetyl-CoA synthetase | [CAS]
9012-31-1 | [Synonyms]
EC 6.2.1.1
ACETATE THIOKINASE
ACETATE: COA LIGASE
ACETYL-COA SYNTHETASE
Synthetase, acetyl coenzyme A
S-ACETYL COENZYME A SYNTHETASE
acetate coa ligase (amp forming)
S-acetyl coenzyme A synthetase*from bakers yeast
ACETATE THIOKINASE: ACETATE: COA LIGASE [AMP FORMING]
Acetate CoA ligase (AMP forming), Acetate thiokinase
Acetyl-coenzyme A synthetase from Bacillus subtilis, Recombinant
s-acetyl-coenzyme a synthetase from baker's yeast (s. cerevisiae)
Native Bakers yeast (S. cerevisiae) S-Acetyl-coenzyme A synthetase
S-Acetyl-coenzyme A, synthetase from baker's yeast (S. cerevisiae) lyophilized powder, ≥3 units/mg protein | [EINECS(EC#)]
232-729-7 | [MDL Number]
MFCD00081267 |
| Hazard Information | Back Directory | [Uses]
S-Acetyl-coenzyme A synthetase may be used to study various metabolic pathways, such as glycolysis, gluconeogenesis, pyruvate metabolism and CO2 fixation. It may also be used in gene expression studies. | [Biochem/physiol Actions]
Acetyl-coenzyme A synthetase catalyzes the production of acetyl-CoA. It is involved in histone acetylation in the nucleus. It may be involved in the growth of nonfermentable carbon sources such as glycerol. Acetyl-coenzyme A synthetase is induced by acetate, acetaldehyde and ethanol . | [Purification Methods]
This enzyme has been purified by extraction into phosphate buffer pH 6.8-7.0 containing 2-mercaptoethanol and EDTA, protamine sulfate precipitation, polyethylene glycol fractionation, Alumina gel filtration, concentration by (NH4)2SO4 precipitation, Bio-Gel A-0.5m chromatography and DEAE-cellulose gradient chromatography. It has Km (apparent) 0.24mM (for acetate) and 0.035mM (for CoA); 1.2 mM for ATP and Mg2+ 4.0mM. [Frenkel & Kitchens Methods Enzymol 71 317 1981.] |
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