| Identification | Back Directory | [Name]
ENOLASE | [CAS]
9014-08-8 | [Synonyms]
ENOLASE
EC 4.2.1.11
EC: 4.2.1.11
ENOLASE USP/EP/BP
ENOLASE FROM YEAST
PHOSPHOPYRUVATE HYDRATASE
enolase from bakers yeast
enolase from rabbit muscle
2-PHOSPHO-D-GLYCERATE HYDRO-LYASE
Enolase, 2-phospho-D-glycerate hydro-lyase
enolase from baker's yeast (s. cerevisiae)
Phosphopyruvate hydratase (from Yeast), 50% glycerol solution
2-Phospho-D-glycerate hydro-lyase, Phosphopyruvate hydratase
Enolasefromrabbitmusclecrystallinesuspensionin3,2Mammoniumsulfate
Enolase from baker's yeast (S. cerevisiae),2-Phospho-D-glycerate hydro-lyase, Phosphopyruvate hydratase
ENOLASE FROM RABBIT MUSCLE CRYSTALLINE SUSPENSION IN 3.2 M AMMONIUM SULFATE.ACTIVITY: ~30 UNITS/MG PROTEIN | [EINECS(EC#)]
232-754-3 | [Molecular Formula]
NULL | [MDL Number]
MFCD00131019 |
| Chemical Properties | Back Directory | [Definition]
An enzyme active in glycolysis that catalyzes the conversion of 2-phosphoglyceric acid to the phosphorylated enol of pyruvic acid. | [storage temp. ]
2-8°C
| [solubility ]
15 mM Tris HCl, pH 7.4: soluble 1.0mg/mL, clear | [form ]
lyophilized powder
| [Specific Activity]
≥50units/mg protein | [CAS DataBase Reference]
9014-08-8 | [EPA Substance Registry System]
Hydratase, phosphoenolpyruvate (9014-08-8) |
| Hazard Information | Back Directory | [Uses]
The enzyme from Sigma has been used as an antigen during ELISA. the study used human granulocyte proteins to identify and characterize autoantibodies against catalase and α-enolase in patients with primary sclerosing cholangitis. It has been used to study temperature- and denaturant-induced yeast enolase denaturation using fourier transform infrared spectroscopy. It has also been used along with other proteins to study gradient chromatofocusing-mass spectrometry; a new technique for protein analysis. | [General Description]
Enolase (2-phosphoglycerate hydratase) is a cytosolic enzyme. This cytosolic metalloenzyme is encoded by three homologous genes in mammals. Enolase the from baker′s yeast is a homodimer containing two bound Mg2+ ions. The peptide consists of 436 amino acids and contains a single cysteine residue and corresponds to a molecular weight of 93 kDa. Two of the active site components include His191 and Arg414. Enolase is seen in archaea, bacteria and eukaryotes. | [Biochem/physiol Actions]
Enolase plays a key role in glycolysis and gluconeogenesis. Enolase, found in the nucleus of mammalian cells plays a key role in transcriptional modulation of genes, which participates in morphological transformation and cell proliferation. |
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| Company Name: |
Sigma-Aldrich
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021-61415566 800-8193336 |
| Website: |
https://www.sigmaaldrich.cn |
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