| Identification | Back Directory | [Name]
T4 DNA LIGASE | [CAS]
9015-85-4 | [Synonyms]
abs60084
DNA LIGASE
EC 6.5.1.1
EC 6.5.1.2
IUB: 6.5.1.1
T4 DNA LIGASE
DNA LIGASE T4
RNA LIGASE, T4 PAHGE
Polynucleotide Ligase
DNA LIGASE, THERMOSTABLE
DNA LIGASE NAD-DEPENDENT
polydeoxyribonucleotide synthase
Synthetase, polydeoxyribonucleotide
T4 dna ligase from T4-infected E. coli
RNA Ligase, T4 pahge, recombinant, Soln.
dna ligase from t4-infected escherichia coli
Synthetase,polydeoxyribonucleotide (adenosine triphosphate) | [EINECS(EC#)]
232-770-0 | [MDL Number]
MFCD00163508 |
| Chemical Properties | Back Directory | [storage temp. ]
−20°C
| [form ]
buffered aqueous glycerol solution
| [biological source]
Escherichia coli (NM 989) | [Specific Activity]
4,000U/mL |
| Hazard Information | Back Directory | [Uses]
Forms an energy dependent phosphodiester linkage between the termini of adjacent polynucleotides of duplex DNA. For ligation of cloning vector and restriction insert fragments | [General Description]
T4 DNA Ligase catalyzes the formation of phosphodiester bonds between neighboring 3′-hydroxyl- and 5′-phosphate ends in double-stranded DNA. Single-stranded nicks in double-stranded DNA are also closed by T4 DNA Ligase.
Contents
T4 DNA Ligase, supplied with 10x concentrated ligation buffer that includes ATP. | [Biological Activity]
T4 DNA Ligase forms an energy dependent phosphodiester linkage between the termini of adjacent polynucleotides of duplex DNA. The ligation reaction requires ATP as a cofactor. Ligation of blunt-ended fragments requires higher enzyme concentration and can be facilitated by using PEG in the reaction mixture. The enzyme requires a 3μ hydroxyl and 5μ phosphate for ligation. Self-ligation of vector DNA can be prevented by dephosphorylation with alkaline phosphatase. T4 ligase plays an active role in repair of DNA and RNA nicks. |
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| Company Name: |
Sigma-Aldrich
|
| Tel: |
021-61415566 800-8193336 |
| Website: |
https://www.sigmaaldrich.cn |
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