| Identification | Back Directory | [Name]
CHONDROITINASE ABC | [CAS]
9024-13-9 | [Synonyms]
EC 4.2.2.4
units/mg solid
CHONDROITINASE ABC
chondroitinase ABC I
CHONDROITIN ABC LYASE
Lyase, chondroitin ABC
Chondroitinase ABC, Recomb.
chondroitinase-abc bsa free
lyophilized powder, 0.3-3
chondroitin-sulfate-ABC endolyase
chondroitinase abc F. proteus vulgaris
Chondroitinase ABC from Proteus vulgaris
Native Proteus vulgaris Chondroitinase ABC
CHONDROITIN ABC LYASE, FROM PROTEUS VULGARIS
Chondroitinase ABC from Proteus vulgaris, Recombinant
Chondroitinase ABC, Proteus vulgaris*Not available Japan*
Chondroitinase ABC from Proteus vulgaris,Chondroitin ABC Lyase | [EINECS(EC#)]
232-777-9 | [Molecular Formula]
NULL | [MDL Number]
MFCD00130795 |
| Chemical Properties | Back Directory | [storage temp. ]
−20°C
| [solubility ]
0.01% bovine serum albumin aqueous (BSA) solution: soluble | [form ]
lyophilized powder
| [color ]
White to off-white | [biological source]
bacterial (Proteus vulgaris) | [Specific Activity]
50-250units/mg protein | [Major Application]
diagnostic assay manufacturing |
| Hazard Information | Back Directory | [Uses]
Chondroitinase ABC from Sigma has been used to digest O-linked chondroitin sulfate-like glycosaminoglycan (CSGAG). The enzyme has also been used to digest glycosaminoglycan chains in proteoglycans during the construction and expression of a recombinant versican gene in mammalian systems. Furthermore, it has been used to digest chondroitin sulfate in the proteoglycan complexes. The enzyme has also been used in the removal of glycan chains from aggrecan, which eliminates the axon growth-inhibitory effect of aggrecan. | [Uses]
The enzyme has been used to study the consequences of inducing acute, long-lasting changes in chondroitin sulfate proteoglycans using adult rat brain cells. Glycosaminoglycans have been digested with chondroitinase ABC for chondroitin/dermatan sulfate quantitation in keratocyte cell cultures obtained from fresh bovine corneal stromae. | [Biochem/physiol Actions]
Chondroitinase ABC catalyzes the eliminative degradation of polysaccharides containing (1-4)-β-D-hexosaminyl and (1-3)-β-D-glucuronosyl (or (1-3)-α-L-iduronosyl) linkages to disaccharides containing 4-deoxy-β-D-gluc-4-enuronosyl groups. It acts on chondroitin 4-sulfate, chondroitin 6-sulfate, and dermatan sulfate. However, it acts slowly on hyaluronate. The molecular weight is approximately 120 kDa with 2 non-identical subunits of molecular masses 86 kDa and 32 kDa. The pH optimum is 8.0 with chondroitin sulfate and 6.8 with hyaluronic acid. The optimal temperature is 37 °C. 1 mM Zn2+ acts as an inhibitor, while 0.05 M acetate is an activator of the enzyme. |
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| Company Name: |
Sigma-Aldrich
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| Telephone: |
021-61415566 800-8193336 |
| Website: |
https://www.sigmaaldrich.cn |
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