ChemicalBook--->CAS DataBase List--->9029-47-4

9029-47-4

9029-47-4 Structure

9029-47-4 Structure
IdentificationBack Directory
[Name]

EC 1.13.11.3
[CAS]

9029-47-4
[Synonyms]

EC 1.13.11.3
PROTOCATECHUATE 3,4-DIOXYGENASE
protocatechuate 3,4-dioxygenase from*pseudomonas
PROTOCATECHUATE 3,4-DIOXYGENASE FROM*PSE UDOMONAS SP
Protocatechuate-3,4-dioxygenase,from Pserdomonas sp.
Native Pseudomonas sp. Protocatechuate 3, 4-dioxygenase
Protocatechuate 3,4-Dioxygenase from Bacteria, Recombinant
rPCO, Protocatechuate: oxygen 3,4-oxidoreductase (decyclizing)
Protocatechuate 3,4-Dioxygenase, recombinant from bacteria, solution
frompseudomonasspecies,lyophilizedpowderactivity:3-5umgsolidcontainsapprox.40
[MDL Number]

MFCD00132132
Chemical PropertiesBack Directory
[storage temp. ]

-20°C
[form ]

lyophilized powder
[color ]

White to off-white
[biological source]

bacterial (Pseudomonas spp.)
[Specific Activity]

≥3units/mg solid
Safety DataBack Directory
[WGK Germany ]

3
Hazard InformationBack Directory
[Uses]

The enzyme has been used to create an oxygen scavenging system along with protocatechuate (PCA) and Trolox. The enzyme employs a nonheme iron center that catalyzes the conversion of PCA and molecular oxygen into β-carboxy-cis,cis-muconic acid, while the antioxidant Trolox suppresses slow blinking and photobleaching of cyanine dyes. It has been used in the preparation of imaging buffer along with DMB-BSA (dynein motility buffer-BSA), ATP and protocatechuate in single molecule motility assay.
[General Description]

Protocatechuate 3,4-Dioxygenase belongs to the non-heme iron family of enzymes. The active site of the enzyme contains Fe3+.
[Biochem/physiol Actions]

Protocatechuate 3,4-Dioxygenase catalyzes the degradation of 3,4-dihydroxybenzoate (protocatechuate) into β-carboxy-cis,cis-muconate.
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