95470-42-1

51605-32-4

95470-42-1

General procedure: The synthesis of ethyl 2-bromo-4-methyl-1H-imidazole-5-carboxylate (EV-AT8648-001) from ethyl 4-methyl-5-imidazolecarboxylate was performed as follows: Step 1: To a stirred solution of ethyl 4-methyl-1H-imidazole-5-carboxylate (CAS 51605-32-4, 500 mg, 3.24 mmol) in a mixture of acetonitrile (10 mL) and chloroform (10 mL), N-bromosuccinimide (577 mg, 3.24 mmol) was added. The reaction was carried out under nitrogen protection with stirring at room temperature for 18 hours. Upon completion of the reaction, the reaction mixture was concentrated and the residue was purified by fast column chromatography (eluent: 10-100% ethyl acetate/heptane gradient) to afford 560 mg (73% yield) of the target product, ethyl 2-bromo-4-methyl-1H-imidazole-5-carboxylate (EV-AT8648-001), as an off-white solid. LCMS analysis (Method D): retention time 0.87 min, mass spectrum m/z = 233/235 ([M+H]+).