1404-90-6
1404-90-6 结构式
物理化学性质
应用领域
常见问题列表
一种万古霉素菌株及其发酵产万古霉素的方法，包括以下步骤：
1）将万古霉素生产菌株进行液体发酵培养基培养，液体发酵种子培养基 组成为酵母粉3～6g/L，麦芽提取物3～5g/L，蛋白胨10～15g/L，葡萄糖 15～20g/L，pH为6.8；种子培养条件为：28～30℃，装液量40/250mL， 摇床转速150～220rpm，培养24～48h；液体发酵培养基组成为糊精100～150g/L，土豆蛋白15～25g/L，黄豆粉15～25g/L，磷酸氢二钾0.1～0.5g/L，消沫剂1～3g/L，氯化钠1.2～1.6g/L，pH 6.8～7.2，培养条件为28～30℃，装液量40/250mL，摇床转速150～220rpm，培养90～130h，所得溶液为 含净抗生素活性洗脱液；
2）在沉淀容器中沉淀步骤1）中所得的含净抗生素活性洗脱液，得到晶体浆料，并且搅拌该晶体浆料，将PH值连续调节至所需程度，直至完全沉淀，得到晶体沉淀物；
3）将步骤2）得到的晶体沉淀物溶解为水溶液，然后通过阴离子交换树脂将其转为万古霉素。
Vancomycin is a large glycopeptide compound with a molecular weight of 1450 Da. Vancomycin is a unique glycopeptide structurally unrelated to any currently available antibiotic. It also has a unique mode of action inhibiting the second stage of cell wall synthesis of susceptible bacteria. Vancomycin is active against a large number of species of Gram-positive bacteria, such as Staphylococcus aureus, Staph. epidermidis, Str. agalactiae, Str. bovis, Str. mutans, viridans streptococci, enterococci .
Vancomycin is administered intravenously, with a standard infusion time of at least 1 h, to minimize infusion-related adverse effects. Subjects with normal creatinine clearance, vancomycin has an α-distribution phase of 30 min to 1 h and a β-elimination half-life of 6-12 h. The volume of distribution is 0.4–1 L/kg. The binding of vancomycin to protein ranges from 10% to 50%. Factors that affect the overall activity of vancomycin include its tissue distribution, inoculum size, and protein-binding effects. Vancomycin treatment of infected mice is associated with improved clinical, diarrhea, and histopathology scores and survival during treatment.