35013-72-0
35013-72-0 结构式
基本信息
(+)生物素-N-琥珀酰亚胺基酯
D-生物素-N-羟基琥珀酰亚胺酯
N-羟基丁二酰亚胺基生物素
N-羟基琥珀酰亚胺基生物素
N-羟基琥珀酰亚胺生物素
(+)-生物素-N-羟基琥珀酰亚胺酯
(+)-生物素-N-羟基琥珀酰亚胺酯
(+)-生物素-N-羟基琥珀酰亚胺酯,98%
BIOTIN (LONG ARM) NHS
BIOTIN-NHS
BIOTIN-N-HYDROXYSUCCINIMIDE
(+)-BIOTIN-N-HYDROXYSUCCINIMIDE ESTER
BIOTIN-N-HYDROXYSUCCINIMIDE ESTER
(+)-BIOTIN N-SUCCINIMIDYL ESTER
BIOTIN-OSU
BIOTIN SE
BIOTIN SUCCINIMIDYL ESTER
BIOTINYL-N-HYDROXY-SUCCINIMIDE
(+)-BIOTINYL-N-SUCCINIMIDYL ESTER
BNHS
D-(+)BIOTIN N-HYDROXYSUCCINIMIDE ESTER
D-BIOTIN-N-HYDROXYSUCCINIMIDE ESTER
D-BIOTIN, SE
D-BIOTIN, SUCCINIMIDYL ESTER
D-BIOTINYL-OSU
NHS-BIOTIN
NHS-D-BIOTIN
物理化学性质
制备方法
6066-82-6
58-85-5
35013-72-0
一般步骤：向D-生物素（0.82 mmol，200 mg）和N-羟基丁二酰亚胺（0.89 mmol，102 mg）的混合溶液中加入3-(乙基亚氨基甲基氨基)-N,N-二甲基丙-1-胺盐酸盐（0.96 mmol，184 mg）。将反应混合物在室温下搅拌过夜后，进行浓缩，得到白色固体。将粗产物与异丙醇混合，加热至70℃后冷却。通过过滤得到纯产物，为白色粉末，收率为91%（0.75 mmol，255 mg）。
参考文献：
[1] Journal of the American Chemical Society, 2007, vol. 129, # 45, p. 13987 - 13996
[2] New Journal of Chemistry, 2013, vol. 37, # 11, p. 3762 - 3769
[3] Organic Letters, 2013, vol. 15, # 16, p. 4130 - 4133
[4] Journal of Materials Chemistry, 2004, vol. 14, # 17, p. 2638 - 2642
[5] Beilstein Journal of Organic Chemistry, 2015, vol. 11, p. 784 - 791
常见问题列表
(+)生物素-N-琥珀酰亚胺基酯可对溶液中的抗体、蛋白质和任何其他含伯胺的大分子进行简单有效的生物素标记。
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Calculations
:
For best results, use ≥ 12-fold molar excess of biotin for a 10 mg/mL protein solution or ≥ 20-fold molar excess of biotin for a 2 mg/mL protein solution.
1.Calculate millimoles of biotin reagent to add to the reaction for a 20-fold molar excess:
mL protein × (mg protein/mL protein) × (mmoL protein/mg protein) × (20 mmoL protein/mmoL protein) = mmoL Biotin.
* 20 = Recommended molar fold excess of biotin for 2 mg/ml protein sample
2. Calculate microliters of 10 mM biotin reagent solution (prepared in Step B.3) to add to the reaction:
mmoL Biotin × (1000000 μL/L) × (L/ 10 mmoL) =μL Biotin
Biotin Labeling Reaction
:
1. Dissolve 1-10 mg protein in 0.5-2.0 mL PBS according to the calculation made in Calculations.
2. Before use, prepare a 10 mM solution of the biotin reagent in an organic solvent such as DMSO.
3. Add the appropriate volume (μL) of 10 mM biotin reagent solution to the protein solution.
4. Incubate reaction on ice for two hours or at room temperature for 30 minutes.
5. To tests the labeled protein by ELISA or Western blot.
(+)生物素-N-琥珀酰亚胺基酯修饰生物分子有助于目标赖氨酸残基在ε-氨基上的生物素化，并识别表面赖氨酸残基。其被用作识别蛋白质与几种生物分子相互作用的工具。